产品名称
Anti-VCAM-1 Antibody, clone M/K, clone M/K - 2, Chemicon®, from rat
biological source
rat
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
M/K - 2, monoclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... L1CAM(3897), VCAM1(7412)
Application
Anti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.
Studies of cell-cell interaction
Cell adhesion assays
Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures
Immunoprecipitation of the VCAM-1 integrin complex
Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.
Optimal working dilutions must be determined by the end user.
Cell adhesion assays
Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures
Immunoprecipitation of the VCAM-1 integrin complex
Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.
Optimal working dilutions must be determined by the end user.
Analysis Note
Control
EOMA and BMS2 endothelial cell lysates
EOMA and BMS2 endothelial cell lysates
Biochem/physiol Actions
Recognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB2242
Physical form
Format: Purified
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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