CBL1300
Anti-VCAM-1 Antibody, clone M/K
clone M/K - 2, Chemicon®, from rat
别名:
CD106
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关于此项目
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
M/K - 2, monoclonal
Application:
FACS, IHC, IP
Citations:
8
biological source
rat
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
M/K - 2, monoclonal
species reactivity
mouse
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... L1CAM(3897), VCAM1(7412)
Application
Anti-VCAM-1 Antibody, clone M/K is an antibody against VCAM-1 for use in FC, IH & IP.
Studies of cell-cell interaction
Cell adhesion assays
Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures
Immunoprecipitation of the VCAM-1 integrin complex
Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.
Optimal working dilutions must be determined by the end user.
Cell adhesion assays
Inhibition of lymphopoieisis in Whitlock-Witte long-term marrow cultures
Immunoprecipitation of the VCAM-1 integrin complex
Detection of VCAM-1 cells by immunofluorescence and immunohistochemical staining: acetone or fresh frozen sections work best; although zinc formalin or PLP fixations are reported to work but they have not be verified by Chemicon.
Optimal working dilutions must be determined by the end user.
Biochem/physiol Actions
Recognizes Murine vascular adhesion molecule (VCAM-1), single chain glycoprotein of Mr 100- kDa (under reducing conditions). VCAM-1 is present on the +/+ 2.4 stromal cell clone, large cells in bone marrow, and a subset of thymic macrophages. The stromal cell antigen is also detectable on the EOMA and BMS2 endothelial cell lines. The antibody reacts with all known isoforms of murine VCAM-1.
Physical form
Format: Purified
Analysis Note
Control
EOMA and BMS2 endothelial cell lysates
EOMA and BMS2 endothelial cell lysates
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: MAB2242
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Michael J Borrett et al.
Cell reports, 32(6), 108022-108022 (2020-08-14)
The transitions from developing to adult quiescent and activated neural stem cells (NSCs) are not well understood. Here, we use single-cell transcriptional profiling and lineage tracing to characterize these transitions in the murine forebrain. We show that the two forebrain
Haowen Qiao et al.
Journal of medical virology, 96(5), e29671-e29671 (2024-05-15)
The coronavirus disease of 2019 (COVID-19) pandemic has led to more than 700 million confirmed cases and nearly 7 million deaths. Although severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) virus mainly infects the respiratory system, neurological complications are widely reported in
Cathy Grutzmacher et al.
American journal of physiology. Renal physiology, 304(1), F19-F30 (2012-10-19)
Diabetic nephropathy is the most common cause of end-stage renal disease and is a major risk factor for cardiovascular disease. In the United States, microvascular complications during diabetic nephropathy contribute to high morbidity and mortality rates. However, the cell-autonomous impact
Elham Khanicheh et al.
PloS one, 8(3), e58761-e58761 (2013-04-05)
Inflammatory changes on the endothelium are responsible for leukocyte recruitment to plaques in atherosclerosis. Noninvasive assessment of treatment-effects on endothelial inflammation may be of use for managing medical therapy and developing novel therapies. We hypothesized that molecular imaging of vascular
Sophie F Ellermann et al.
Frontiers in pharmacology, 13, 992262-992262 (2022-12-20)
Major surgery induces systemic inflammation leading to pro-inflammatory activation of endothelial cells. Endothelial inflammation is one of the drivers of postoperative organ damage, including acute kidney injury Tumour Necrosis Factor alpha (TNF-α) is an important component of surgery-induced pro-inflammatory activation
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