Anti-CD62E/CD62P Antibody, clone 1.2B6, FITC Conjugate

65.51 kDa (CD62E) and 89.70 kDa (CD62P) calculated.

Selectins (CD62s) are single-chain transmembrane glycoproteins that function as cell adhesion molecules (CAMs) by interacting with a range of oligosaccharide structures including sialyl-Lewis X (sLeX) via their C-type lectin domain. All three known selectins (L-, E-, and P-selectin or CD62L CD62E, CD62P, respectively) share a similar overall domain structure that includes an N-terminal C-type lectin domain (calcium-dependent lectin domain), an EGF-like domain, a variable number of short consensus repeat (SCR) or Sushi domains (2, 6, and 9 for CD62L CD62E, CD62P, respectively), a transmembrane domain, and a cytoplasmic tail. The extracellular domains show a high homology among the three selectins, the transmembrane and cytoplasmic domains however are not conserved among the three. E-selectin (CD62E, ELAM-1) and P-selectin (CD62P, GMP-140, LECAM3, PADGEM) are mainly expressed on endothelial cells at inflammatory sites, whereas L-selectin (CD62L) is exclusively expressed on leukocytes.

Epitope: C-type lectin domain.

TNF-activated human umbilical vein endothelial cells.

Anti-CD62E/CD62P Antibody, clone 1.2B6, FITC Conjugate is an antibody against CD62E/CD62P for use in Immunofluorescence, Flow Cytometry.

Flow Cytometry Analysis: A representative lot detected E-selectin (CD62E) expression induction on the surface of human umbilical vein endothelial cells (HUVECs) following stimuation with a trimeric form of the murine CD40 ligand, CD40LT (Karmann, K., et al. (1995). Proc. Natl. Acad. Sci. U. S. A. 92(10):4342-4346).
The unconjugated antibody (Cat. Nos. CBL180 & CBL180-I) is shown to be suitable also for ELISA, flow cytometry, immunohistochemistry, immunoprecipitation, neutralization, and Western blotting applications.

Research Category
Cell Structure

Research Sub Category
Adhesion (CAMs)

The antibody is specific to human antigen E-selectin (ELAM-1, 115 kDa) and P-selectin that are expressed on activated endothelial cells. The ligand is expressed on neutrophils and may function as an early part in neutrophil binding to the endothelium. The antibody inhibits neutrophil adhesion to E-selectin and P-selectin when used as F(ab′)2 fragments. Although previously thought to recognize only human CD62E, later data (Goda, K., et al., (1999). Eur. J. Immunol. 29(5):1551-1560) shows that this antibody also recognizes human CD62P binding to a common epitope on these members of the selectin family.

Protein G Purified

Purified mouse monoclonal IgG1κ antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Immunofluorescence in human tonsil tissue.

Immunofluorescence Analysis: A 1:50 dilution of this antibody detected CD62E/CD62P in human tonsil tissue sections.

Concentration: Please refer to lot specific datasheet.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.