Human Integrin αVβ5 Protein, Triton X-100 Formulation

Integrin alphaV beta5 was purified from human placenta by affinity chromatography using immobilized monoclonal antibodies to alphaV beta5 integrin. Two bands are identified with silver stain, corresponding to alphaV (145kDa) and beta5 (90kDa) subunits under nonreducing conditions. Product was tested and found negative for HIV, HBsAg, syphilis, and hepatitis. Integrin alphaV beta5 interacts with vitronectin in ELISA.

Electrophoresis

Immunoblotting (nonreduced conditions)

Ligand Binding studies.

Optimal working dilutions must be determined by end user.

Purified protein in 20 mM Tris-HCl, pH 7.5, 150 mM NaCl, 2 mM MgCl, 0.2% Triton X-100, with no preservatives.

Maintain at -70°C in undiluted aliquots. Avoid repeated freeze/ thaw cycles.

Two main protein bands corresponding to aV (145 kD) and β5 (90 kD) subunits are seen in silver stained gel under non-reduced conditions.

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