biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
DM1A, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
human, mouse, gerbil, chicken, rat
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
dilution
(Immunoblotting (20-100 ng/mL, colorimetric or chemiluminescence;
Immunofluorescence (5 µg/mL))
isotype
IgG1
shipped in
wet ice
Quality Level
Gene Information
human ... TUBA1A(7846)
target post-translational modification
unmodified
General description
识别在全部真核细胞中发现的~60 kDa微管蛋白蛋白。可用作阳性对照。
该抗α微管蛋白小鼠mAb(DM1A)经验证可用于免疫印迹和免疫荧光检测α微管蛋白。
通过用指定的免疫原免疫BALB/c57小鼠并将脾细胞与NS-1小鼠骨髓瘤融合而产生的纯化的小鼠单克隆抗体。识别〜60 kDa微管蛋白。
Immunogen
鸡
天然鸡脑微管
Application
免疫印迹(20-100 ng/ml,比色或化学发光;请参阅应用参考)
免疫荧光(5 µg/ml)
免疫荧光(5 µg/ml)
Packaging
请参考特定浓度批号的标签。
Physical form
溶于50 mM磷酸钠缓冲液,含0.2%明胶。
Analysis Note
阳性对照
任何细胞系
任何细胞系
阴性对照
trp E(Ab-1)
trp E(Ab-1)
Other Notes
Blose, S.H., et al. 1984.J. Cell.Biol.98, 847.
Lin, J.J.C., et al. 1984.In: Monoclonal antibodies and functional cell lines.Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds.Plenum Publishing Corp.
Lin, J.J.C.1982.Cold Spring Harbor Symp. on Quant.Biol.46, 769.
Goldman, R.D., et al. 1979.Ann.Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.)1976.Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
Lin, J.J.C., et al. 1984.In: Monoclonal antibodies and functional cell lines.Kennett, R.H., Bechtol, K.B. and McKearn, T.J., eds.Plenum Publishing Corp.
Lin, J.J.C.1982.Cold Spring Harbor Symp. on Quant.Biol.46, 769.
Goldman, R.D., et al. 1979.Ann.Rev. Physiol.41, 701.
Goldman, R., Pollard, T. and Rosenbaum, J. (eds.)1976.Cold Spring Harbor Conference on Cell Proliferation, Books A-C, Cold Spring Harbor, New York.
微管蛋白存在于全部细胞中,用作免疫荧光或免疫印迹的阳性对照。在单个系统中,应对抗体进行滴定以获得最佳结果。
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
毒性:标准处理(A)
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存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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