species reactivity
rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable
storage temp.
2-8°C
Application
The rat C-reactive protein (CRP) kit is designed to measure the amount of rat CRP in serum or plasma. There are enough reagents included in this kit for one 96-well immuno-assay plate. We recommend running duplicate wells for samples and standards.
For Research Use Only; Not for use in diagnostic procedures.
For Research Use Only; Not for use in diagnostic procedures.
Biochem/physiol Actions
Used to detect/quantify: C-Reactive Protein
Disclaimer
For research use only. Not for use in diagnostic procedures.
Features and Benefits
- Test tubes for diluting samples
- Precision pipettes to deliver 2--1000 mL
- Microplate reader with 450 ± 10 nm filter
- Software package facilitating data generation and analysis
- Microtitration plate washer (optional) [Manual washing is possible but not preferable.]
- Absorbent material for blotting the microwell plate
- Clean 250-500 mL wash bottle for buffer
- Adhesive cover for microplates
- Deionized (distilled) water
General description
The rat C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures rat CRP.
Test Principle
With the rat CRP ELISA, standards, quality controls and samples of sera are incubated in microtitration wells coated with polyclonal anti-rat CRP antibody. After a thorough wash, polyclonal anti-rat CRP antibody labelled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody-CRP complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450 nm. The absorbance is proportional to the concentration of CRP. A standard curve is constructed by plotting absorbance values versus CRP concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
Test Principle
With the rat CRP ELISA, standards, quality controls and samples of sera are incubated in microtitration wells coated with polyclonal anti-rat CRP antibody. After a thorough wash, polyclonal anti-rat CRP antibody labelled with horseradish peroxidase (HRP) is added to the wells and incubated with the immobilized antibody-CRP complex. Following another washing step, the remaining HRP-conjugated antibody is allowed to react with the substrate and tetramethylbenzidine. The reaction is stopped by addition of acidic solution, and absorbance of the resulting yellow color product is measured spectrophotometrically at 450 nm. The absorbance is proportional to the concentration of CRP. A standard curve is constructed by plotting absorbance values versus CRP concentrations of standards, and concentrations of unknown samples are determined using this standard curve.
Other Notes
- Microwell Strips (96 wells), coated with Anti-rat CRP Antibody, vacuum sealed (Catalog No. CYT294a)
- Conjugate Concentrate (100x) (Anti-rat CRP Antibody, Horseradish Peroxidase Conjugate), 0.12 mL (Catalog No. CYT294b)
- Rat CRP Standard, 1.33 mg/mL (10x), 0.25 mL (Catalog No. CYT294c)
- Substrate (TMB) Solution, 12 mL, Protect from light. Non-carcinogenic. (Catalog No. CYT294d)
- Stop Solution (diluted phosphoric acid), 12 mL (Catalog No. CYT294e)
- Wash Buffer, 1 packet (PBS with 0.05% Tween 20, pH 7.4 when reconstituted to 1 liter of distilled water) (Catalog Number CYT294f)
Packaging
96 wells
Preparation Note
Store the kit at 2° to 8°C for up to 6 months after date of receipt or until expiration date.
Precautions
Precautions
- Avoid contact with the acidic Stop Solution and Substrate Solution, which contains urea peroxide. Wear gloves and eye protection when handling these reagents. In case of contact with the Stop Solution and the Substrate Solution wash skin thoroughly with water and seek medical attention, when necessary.
- HRP-labeled conjugate and TMB-substrate are photosensitive and are packaged in a protective opaque bottle. Store in the dark and return to storage after use.
- Wear gloves and laboratory coats when handling the kit.
- The materials must not be pipetted by mouth.
- Do not drink, eat or smoke in the areas where the kit is being handled.
- Reagents with different lot numbers should not be mixed.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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