生物来源
goat
质量水平
抗体形式
affinity isolated antibody
抗体产品类型
secondary antibodies
克隆
polyclonal
表单
lyophilized
不包含
preservative
种属反应性
rabbit
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
dilution
(ELISA (1:5000-1:100,000)
Immunoblotting (1:5000-1:100,000, colorimetric; 1:10,000-1:200,000, chemiluminescence)
Immunohistochemistry (1:500-1:5000))
同位素/亚型
IgG
运输
ambient
储存温度
2-8°C
靶向翻译后修饰
unmodified
一般描述
免疫亲和纯化的山羊多克隆抗体,吸附人血清蛋白并与辣根过氧化物酶偶联。可识别兔IgG。
经验证,该山羊抗兔IgG过氧化物酶偶联物可用于ELISA和免疫组化以检测兔IgG。
应用
ELISA(1:5000-1:100,000)
免疫印迹(1:5000-1:100,000,比色;1:10,000-1:200,000,化学发光)
免疫组织化学(1:500-1:5000)
免疫印迹(1:5000-1:100,000,比色;1:10,000-1:200,000,化学发光)
免疫组织化学(1:500-1:5000)
外形
从250 mM NaCl,10 mM磷酸钠,15 mg/ml BSA(pH 7.6)中冻干。
制备说明
用1.5 ml H₂O复溶抗体。由于充分混合会使抗体变性,因此在4°C下轻轻混合使其溶解。在复溶后分装并冷冻保存(-70°C)。避免溶液反复冻融。或者,可以添加甘油(最终浓度为50%),然后将复溶的抗体储存于-20°C。请注意,在这些条件下,抗体和缓冲盐的浓度将为原始浓度的一半。
其他说明
为了确保这些试剂的质量,每个批次都使用我们的几种一抗进行测试。通过免疫电泳检测抗体特异性,结果显示对兔IgG和大多数兔免疫球蛋白常见的轻链具有特异性。未检测到与非免疫球蛋白血清蛋白的交叉反应性。该抗体也已通过ELISA和/或吸附进行了检测,以确保与人血清蛋白的交叉反应性降至最低,但该抗体可能与其他物种的免疫球蛋白具有交叉反应性。在单个系统中,应对抗体进行滴定以获得最佳结果。
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
毒性:标准处理(A)
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储存分类代码
11 - Combustible Solids
WGK
WGK 3
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