biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
F4, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
polyoma-infected cells
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
2-8°C
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~100 kDa large T antigen, the ~55 kDa middle T antigen, and the ~22 kDa small T antigen of polyoma virus.
Recognize polyoma virus large, middle, and small T antigens.
This Anti-Polyoma Virus T Antigen (Ab-4) Mouse mAb (F4) is validated for use in Immunofluorescence, Immunoprecipitation, Immunoblotting for the detection of Polyoma Virus T Antigen (Ab-4).
Immunogen
Polyoma virus T antigen
Application
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1-2 µg/ml)
Immunoblotting (not recommended)
Immunoprecipitation (1-2 µg/ml)
Immunoblotting (not recommended)
Physical form
Lyophilized from a volatile buffer, 100 µg BSA.
Preparation Note
Resuspend the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibody should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles of solutions.
Analysis Note
Positive Control
Polyoma infected 3T6 cells
Polyoma infected 3T6 cells
Other Notes
Bolen, J.B., et al. 1984. Cell38, 767.
Courtneidge, S.A. and Smith A.E. 1984. EMBO J.3, 585.
Courtneidge, S.A. and Smith, A.E. 1983. Nature303, 435.
Eckhart, W. 1981. Adv. Cancer Res.35, 1.
Ito, Y., et al. 1980. J. Virol.35, 219.
Ito, Y. and Spurr, N. 1980. Cold Spring Harbor Symp. Quant. Biol.44, 149.
Turler, H. 1980. Mol. Cell. Biochem.32, 63.
Smith, A.E., et al. 1979. Cell18, 915.
Eckhart, W., et al. 1979. Cell18, 925.
Schaffhausen, B.S. and Benjamin, T.L. 1979. Cell18, 935.
Courtneidge, S.A. and Smith A.E. 1984. EMBO J.3, 585.
Courtneidge, S.A. and Smith, A.E. 1983. Nature303, 435.
Eckhart, W. 1981. Adv. Cancer Res.35, 1.
Ito, Y., et al. 1980. J. Virol.35, 219.
Ito, Y. and Spurr, N. 1980. Cold Spring Harbor Symp. Quant. Biol.44, 149.
Turler, H. 1980. Mol. Cell. Biochem.32, 63.
Smith, A.E., et al. 1979. Cell18, 915.
Eckhart, W., et al. 1979. Cell18, 925.
Schaffhausen, B.S. and Benjamin, T.L. 1979. Cell18, 935.
For immunoprecipitation, label by autophosphorylation. Antibody should be titrated for optimal results in individual sample types.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
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存储类别
10-13 - German Storage Class 10 to 13
R Novosyadlyy et al.
Oncogene, 28(39), 3477-3486 (2009-07-21)
Polyoma virus middle T antigen (PyVmT) is a powerful viral oncogene; however, the mechanisms of PyVmT activation are poorly understood. The insulin-like growth factor I receptor (IGF-IR) and the insulin receptor (IR) are known to be implicated in the development
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