DR1045
Anti-GCLC Mouse mAb (3H1)
liquid, clone 3H1, Calbiochem®
别名:
Anti-Glutamate-Cysteine Ligase Catalytic Subunit
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关于此项目
UNSPSC Code:
12352203
Clone:
3H1, monoclonal
Species reactivity:
human
Application:
—
Citations:
-
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
3H1, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... GCLC(2729)
General description
Anti-GCLC, mouse monoclonal, clone 3H1, recognizes the ~70 kDa GCLC protein in HeLa and A431 cells. It is validated for use in ELISA, Western blotting, and immunocytochemistry.
Purified mouse monolconal antibody. Recognizes the ~70 kDa GCLC protein.
Recognizes the ~70 kDa GCLC protein in HeLa and A431 cells.
Immunogen
A recombinant polypeptide corresponding to amino acids 528-638 of human GCLC, expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 µg/ml)
Immunocytochemistry (10 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
A431 cells, HeLa cells
A431 cells, HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
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