biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
6E3, monoclonal
form
liquid
does not contain
preservative
species reactivity
human, mouse
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
dilution
(ELISA
Immunoblotting (1-5 µg/mL)
Paraffin Sections (0.2 µg/mL))
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... SALL4(57167)
mouse ... Sall4(99377)
General description
Purified mouse monoclonal antibody. Recognizes the ~100 kDa SALL4 protein.
Recognizes the ~100 kDa SALL4 protein in HeLa and NIH3T3 cells and human testes tissue.
This Anti-SALL4 Mouse mAb (6E3) is validated for use in ELISA, Immunoblotting, Paraffin Sections for the detection of SALL4.
Immunogen
A recombinant polypeptide corresponding to amino acids 954-1054 of human SALL4, expressed as a GST fusion protein
NA
Application



ELISA (see comments)
Immunoblotting (1-5 g/ml)
Paraffin Sections (0.2 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Positive Control
HeLa cells, NIH3T3 cells, Human testes tissue
HeLa cells, NIH3T3 cells, Human testes tissue
Other Notes
Cauffman, G., et al. 2009. Hum. Reprod.24 63.
Forte, A., et al. 2009. Mol. Med.15 392.
Forte, A., et al. 2009. Mol. Med.15 392.
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 µg/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
Yanni Ma et al.
The EMBO journal, 34(3), 361-378 (2014-12-19)
MicroRNAs play important roles in controlling the embryonic stem cell (ESC) state. Although much is known about microRNAs maintaining ESC state, microRNAs that are responsible for promoting ESC differentiation are less reported. Here, by screening 40 microRNAs pre-selected by their
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| DR1052-100UG | 04055977225983 |