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Merck
CN

DR1089

Anti-NKX2-5 Mouse mAb (1E4-G5)

liquid, clone 1E4-G5, Calbiochem®

别名:

Anti-NK2 Transcription Factor Related 5

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关于此项目

UNSPSC Code:
12352203
Clone:
1E4-G5, monoclonal
Species reactivity:
human
Application:
Citations:
-
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biological source

mouse

antibody form

purified antibody

antibody product type

primary antibodies

clone

1E4-G5, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze, avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

Gene Information

human ... NKX2-5(1482)

General description

Purified mouse monoclonal antibody. Recognizes the ~25-35 kDa NKX2-5 protein.
Recognizes the ~25-35 kDa NKX2-5 protein in HeLa cells and human thyroid tissue.
This Anti-NKX2-5 Mouse mAb (1E4-G5) is validated for use in ELISA, Immunoblotting for the detection of NKX2-5.

Immunogen

Full-length, recombinant, human NKX2-5 (aa 1-325), expressed as a GST fusion protein

Application



ELISA (see comments)
Immunoblotting (1-5 g/ml)

Physical form

In PBS, pH 7.2.

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
293T cells
Positive Control
Human thyroid tissue, HeLa cells

Other Notes

For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Yadava, R,S., et al. 2008. Nat. Genet.40 61.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Regulatory Review (Z)

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存储类别

12 - Non Combustible Liquids

wgk

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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