biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
1E4-G5, monoclonal
form
liquid
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze, avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... NKX2-5(1482)
General description
Purified mouse monoclonal antibody. Recognizes the ~25-35 kDa NKX2-5 protein.
Recognizes the ~25-35 kDa NKX2-5 protein in HeLa cells and human thyroid tissue.
This Anti-NKX2-5 Mouse mAb (1E4-G5) is validated for use in ELISA, Immunoblotting for the detection of NKX2-5.
Immunogen
Full-length, recombinant, human NKX2-5 (aa 1-325), expressed as a GST fusion protein
Application
ELISA (see comments)
Immunoblotting (1-5 g/ml)
Physical form
In PBS, pH 7.2.
Preparation Note
Following initial thaw, aliquot and freeze (-20°C).
Analysis Note
Negative Control
293T cells
293T cells
Positive Control
Human thyroid tissue, HeLa cells
Human thyroid tissue, HeLa cells
Other Notes
For ELISA, this antibody can be used as the capture antibody; recommended concentration is 10 ng/ml, 1 µg/well, 100 µl total volume. Variables associated with assay conditions will dictate the proper working dilution.
Yadava, R,S., et al. 2008. Nat. Genet.40 61.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Regulatory Review (Z)
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存储类别
12 - Non Combustible Liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| DR1089-100UG | 04055977226119 |