ECM506
QCM趋化细胞迁移分析,24孔(5 µm),比色法
This 3 um QCM Chemotaxis Assay 24-well plate -colorimetric is performed in a Migration Chamber, based on the Boyden chamber principle.
别名:
细胞迁移实验, 趋化实验
质量水平
种属反应性(根据同源性预测)
all
制造商/商品名称
Chemicon®
QCM
技术
activity assay: suitable
cell based assay: suitable
检测方法
colorimetric
运输
wet ice
一般描述
介绍
细胞迁移是正常细胞过程的基本功能,包括胚胎发育,血管生成,伤口愈合,免疫反应和炎症(1、2)。 大多数细胞大小在30至50 µm之间,可以通过3至10 µm的孔迁移。微孔膜插入物广泛用于细胞迁移和侵袭测定。 其中最广泛接受的是Boyden室测定法。 Boyden室系统使用空心塑料室,一端用多孔膜密封。 该腔室混悬在可能包含培养基和/或化学引诱剂的较大孔上。 将细胞放置在腔室内,并使其通过孔迁移到膜的另一侧。 然后将迁移细胞染色并计数。大多数迁移试验使用8 µm孔径,因为这适用于大多数细胞类型,例如上皮细胞和成纤维细胞。 Millipore 5 µm QCM趋化性试验24孔比色法使用5 µm孔径,该孔径适用于成纤维细胞或癌症细胞亚群,例如NIH-3T3和MDA-MAB 231细胞。通过结晶紫(一种核酸染料)测量向化学引诱剂迁移的细胞,并通过分光光度计进行定量。 该系统可适用于研究不同类型的细胞迁移,包括趋触性、随机迁移和趋化因子。
Millipore QCM 5 µm趋化性测定24孔比色法提供了一种快速有效的系统,可定量测定细胞迁移的各种因素,包括筛选药理剂,评估负责细胞迁移的整联蛋白或其他粘连受体,或分析转染细胞中的基因功能。
此外,Chemicon继续提供许多迁移,侵袭和粘连产品,包括:
• QCM 8µm 24孔趋化性细胞迁移试验(ECM508,509)
• QCM 5µm 24孔趋化性细胞迁移试验- 荧光法(ECM507)
• QCM 8µm 96-孔趋化性细胞迁移试验(ECM510)
• QCM 5µm 96-孔趋化性细胞迁移试验(ECM512)
• QCM 3µm 96-孔趋化性细胞迁移试验(ECM515)
• QCM 96-孔ECMatrix 细胞侵袭试验(ECM555)
• QCM 96孔胶原基细胞侵袭试验(ECM556)
应用
Millipore 5 µm QCM趋化性测定24孔比色法仅用于研究用途;不适用于诊断应用。
包装
制备说明
其他说明
细胞染色剂:(部件号90144)一瓶20 ml。
提取缓冲液:(部件号90145)一瓶20 ml。
棉签:(部件号10202)各50个。
镊子:(部件号10203)各一件。
法律信息
免责声明
警示用语:
Danger
危险声明
危险分类
Eye Irrit. 2 - Flam. Liq. 2
储存分类代码
3 - Flammable liquids
闪点(°F)
53.6 °F
闪点(°C)
12 °C
相关内容
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