biological source
human
assay
>95% (SDS-PAGE and HPLC)
specific activity
≥1 x 105 U/mg
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
impurities
<0.1 ng/μg Endotoxin (of KGF; 1EU/μg)
input
sample type epithelial cells
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
human ... FGF7(2252)
General description
Keratinocyte Growth Factor (KGF or FGF-7) is a potent mitogen for keratinocytes and epithelial cells. Human KGF is a 18.9 kDa protein containing 163 amino acid residues.
Product Source: E.coli
Biochem/physiol Actions
The ED50 was determined by the dose-dependent stimulation of thymidine uptake by KGF-responsive BaF3 cells is ≤ 10 ng/mL
Physical form
Lyophilized from 15 mM Sodium Phosphate pH 7.0 + 0.25 M NaCl.
Preparation Note
The lyophilized powder, though stable for a few weeks at room temperature, is best stored at -20°C. After a quick spin, reconstitute in PBS to a concentration of 0.5-1.0 mg/mL. This solution can be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use. Reconstituted KGF should be stored in undiluted aliquots at -20°C. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
11 - Combustible Solids
wgk
WGK 1
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