产品名称
Granulocyte-Macrophage Colony-Stimulating Factor Protein, Recombinant mouse, The Granulocyte Macrophage Colony Stimulating Factor protein (GM-CSF protein) is a potent species specific stimulator of bone marrow cells.
biological source
mouse
assay
>98% (SDS-PAGE and HPLC)
specific activity
≥5 x 106 U/mg
manufacturer/tradename
Chemicon®
technique(s)
cell culture | mammalian: suitable
input
sample type hematopoietic stem cell(s)
NCBI accession no.
UniProt accession no.
shipped in
dry ice
Gene Information
mouse ... IL3(16187)
Analysis Note
Specific Activity: Chemicon′s mouse GM-CSF is fully biologically active when compared to standards.The ED50 as determined by the dose-dependent stimulation of the proliferation of mouse FDC-P1 cells in ≤ 0.2 ng/mL
Application
For most in vitro applications, murine GM-CSF exerts its biological activity in the concentration range of 0.05 to 0.5 ng/mL. Responding cells are (partial list): Certain BM precursor cells
General description
Granulocyte Macrophage Colony Stimulating Factor (GM-CSF) is a potent species specific stimulator of bone marrow cells. GM-CSF stimulated precursor cells of granulocytes, macrophages, and eosinophils. Murine GM-CSF is a 14.0 kDa protein containing 124 amino acid residues.
Physical form
Sterile filtered solution lyophilized from 10mM HoAc.
Preparation Note
The lyophilized murine GM-CSF, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute in water to a concentration of 100 ng/uL. This solution can be diluted into other buffered solutions. Reconstituted murine GM-CSF should be stored in working aliquots at -20°C.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
11 - Combustible Solids
wgk
WGK 1
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