产品名称
Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3), liquid, clone 134A-2B3, Calbiochem®
biological source
mouse
antibody form
purified antibody
antibody product type
primary antibodies
clone
134A-2B3, monoclonal
form
liquid
contains
≤0.1% sodium azide as preservative
species reactivity
rat, human
manufacturer/tradename
Calbiochem®
storage condition
do not freeze
isotype
IgG1
shipped in
wet ice
storage temp.
2-8°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... TGFA(7039)
Analysis Note
Negative Control
NIH3T3 cells
NIH3T3 cells
Positive Control
A2058 or ST-FeSV FRE cells
A2058 or ST-FeSV FRE cells
Application
Immunoblotting (1-5 µg/ml, see application references)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with P3 x 63 Ag 8.653 mouse myeloma cells (see application references). Recognizes the ~6 kDa denatured and native forms of TGFα protein.
Recognizes the ~6 kDa TGF-α protein in A2058 and ST-FeSVFRE cells.
This Anti-TGF-α (Ab-1) Mouse mAb (134A-2B3) is validated for use in Immunoblotting for the detection of TGF-α (Ab-1).
Immunogen
Human
recombinant, human TGF-α (~6 kDa mature form)
Other Notes
Sorvillo, J.M., McCormack, E.S. 1990. Oncogene5, 377.
Coffey, R.J., et al. 1987. Nature328, 817.
Derynck, R. 1986. J. Cell. Biochem.32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem.261, 14408.
Lee, D.C., et al. 1985 Mol. Cell. Biol.5, 3644.
Kimball, E.S., et al. 1984. Cancer Res.44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.
Coffey, R.J., et al. 1987. Nature328, 817.
Derynck, R. 1986. J. Cell. Biochem.32, 203.
Samsoondor, J., et al. 1986. J. Biol. Chem.261, 14408.
Lee, D.C., et al. 1985 Mol. Cell. Biol.5, 3644.
Kimball, E.S., et al. 1984. Cancer Res.44, 3613.
Marquardt, H., et al. 1983. Proc. Natl. Acad. Sci. USA80, 4684.
Reynolds, F.H., Jr., et al. 1983. Nature292, 259.
Todaro, G.J., et al. 1983. In Genes and Proteins in Oncogenesis, Vogel, H. and Weinstein, I.B. (Eds.) New York, Raven Press 165.
This antibody shows no cross-reactivity with EGF. Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Physical form
In 50 mM sodium phosphate buffer, 0.2% gelatin.
Preparation Note
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) or aliquot and freeze (-20°C).
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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存储类别
10 - Combustible liquids
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法规信息
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