生物来源
mouse
质量水平
抗体产品类型
primary antibodies
克隆
236-5.2, monoclonal
表单
liquid
不包含
preservative
种属反应性
human, mouse, rat
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG1
运输
wet ice
储存温度
−20°C
基因信息
human ... TGFB3(7043)
一般描述
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3x63 Ag 8.653 myeloma cells. Recognizes TGF-β2 and TGF-β3.
Recognizes TGF-β2 and TGF-β3 in umbilical cord cells. Does not cross-react with TGF-β1.
This Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of TGF-β3 (Ab-1).
免疫原
Epitope: within the N-terminal region
recombinant, human TGF-β3
应用
Frozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)
包装
Please refer to vial label for lot-specific concentration.
分析说明
Positive Control
Recombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells
Recombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells
其他说明
Burton, P.B.J., et al. 1993 Int. J. Exp. Path.74, 87.
Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol]327, 145.
ten Dijke, P., et al. 1990 Mol. Cell. Biol.10, 4473.
ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA85, 4715.
de Martin, R., et al. 1987 EMBO J.6, 3677.
Sporn, M.B., et al. 1987 J. Cell Biol.105, 1039.
Derynck, R., et al. 1985 Nature316, 701.
Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA80, 6264.
Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA77, 5258.
Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol]327, 145.
ten Dijke, P., et al. 1990 Mol. Cell. Biol.10, 4473.
ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA85, 4715.
de Martin, R., et al. 1987 EMBO J.6, 3677.
Sporn, M.B., et al. 1987 J. Cell Biol.105, 1039.
Derynck, R., et al. 1985 Nature316, 701.
Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA80, 6264.
Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA77, 5258.
Does not cross-react with TGF-β1. For staining paraffin sections, no pre-treatment is required, but the addition of 0.05% Triton™ X-100 detergent in the incubation buffer is recommended. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
免责声明
Toxicity: Standard Handling (A)
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