产品名称
Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2), liquid, clone 236-5.2, Calbiochem®
biological source
mouse
antibody product type
primary antibodies
clone
236-5.2, monoclonal
form
liquid
does not contain
preservative
species reactivity
human, mouse, rat
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
avoid repeated freeze/thaw cycles
isotype
IgG1
shipped in
wet ice
storage temp.
−20°C
Quality Level
Gene Information
human ... TGFB3(7043)
Analysis Note
Positive Control
Recombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells
Recombinant TGF-β3 protein (Cat. No. PF018) or umbilical cord cells
Application
Frozen Sections (1-5 µg/ml)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)
Immunoblotting (1-5 µg/ml)
Immunoprecipitation (1-2 µg/sample)
Paraffin Sections (1-10 µg/ml, no pre-treatment required, see comments)
Disclaimer
Toxicity: Standard Handling (A)
General description
Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with P3x63 Ag 8.653 myeloma cells. Recognizes TGF-β2 and TGF-β3.
Recognizes TGF-β2 and TGF-β3 in umbilical cord cells. Does not cross-react with TGF-β1.
This Anti-TGF-β3 (Ab-1) Mouse mAb (236-5.2) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of TGF-β3 (Ab-1).
Immunogen
Epitope: within the N-terminal region
recombinant, human TGF-β3
Other Notes
Burton, P.B.J., et al. 1993 Int. J. Exp. Path.74, 87.
Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol]327, 145.
ten Dijke, P., et al. 1990 Mol. Cell. Biol.10, 4473.
ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA85, 4715.
de Martin, R., et al. 1987 EMBO J.6, 3677.
Sporn, M.B., et al. 1987 J. Cell Biol.105, 1039.
Derynck, R., et al. 1985 Nature316, 701.
Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA80, 6264.
Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA77, 5258.
Roberts, A.B., et al. 1990 Philos. Trans. R. Soc. Lond. [Biol]327, 145.
ten Dijke, P., et al. 1990 Mol. Cell. Biol.10, 4473.
ten Dijke, P., et al. 1988 Proc. Natl. Acad. Sci. USA85, 4715.
de Martin, R., et al. 1987 EMBO J.6, 3677.
Sporn, M.B., et al. 1987 J. Cell Biol.105, 1039.
Derynck, R., et al. 1985 Nature316, 701.
Anzano, M.A., et al. 1983 Proc. Natl. Acad. Sci. USA80, 6264.
Todaro, G.J., et al. 1980 Proc. Natl. Acad. Sci. USA77, 5258.
Does not cross-react with TGF-β1. For staining paraffin sections, no pre-treatment is required, but the addition of 0.05% Triton™ X-100 detergent in the incubation buffer is recommended. Antibody should be titrated for optimal results in individual systems.
Packaging
Please refer to vial label for lot-specific concentration.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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