Anti-VEGF (Ab-3) Mouse mAb (14-124)

Purified mouse monoclonal antibody generated by immunizing Balb/c mice with the specified immunogen and fusing splenocytes with mouse SP2/0 cells. Recognizes the ~31-50 kDa VEGF₁₂₁, VEGF₁₆₅, VEGF₁₈₉, and VEGF₂₀₆ proteins.

Recognizes the ~31-50 kDa VEGF₁₂₁, VEGF₁₆₅, VEGF₁₈₉, and VEGF₂₀₆ proteins.

This Anti-VEGF (Ab-3) Mouse mAb (14-124) is validated for use in Immunoblotting, Paraffin Sections for the detection of VEGF (Ab-3).

Vascular endothelial growth factor (VEGF), also known as vascular permeability factor (VPF), is a secreted heparin binding cytokine expressed in tumor and animal cells. VEGF has been implicated in the control of angiogenesis due to its selective mitogenic stimulation of vascular endothelial cells and enhancement of vascular permeability. Other effects of VEGF on vascular endothelial cells include; increasing intracellular calcium, stimulating inositol triphosphate formation, induction of von Willebrand factor release, and stimulating tissue factor expression. VEGF is a ~46 kDa dimer of two identical disulfide-linked subunits existing in four molecular species; 121, 165, 189, and 206 amino acid residues long.

a synthetic peptide corresponding to amino acids from the N-terminus of human VEGF₁₆₅

Immunoblotting (0.5 μg/ml, see comments and application references)
Paraffin Sections (5.0 μg/ml, trypsin or pressure cooker pre-treatment required)

In 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.

Negative Control
Normal brain

Positive Control
Recombinant VEGF protein or fetal kidney

For immunoblotting, include 1% BSA with the primary antibody. Antibody should be titrated for optimal results in individual systems.