生物来源
mouse
质量水平
抗体产品类型
primary antibodies
克隆
CT-3, monoclonal
表单
liquid
包含
≤0.1% sodium azide as preservative
种属反应性
mouse, human, rat
制造商/商品名称
Calbiochem®
储存条件
OK to freeze
avoid repeated freeze/thaw cycles
同位素/亚型
IgG1
运输
wet ice
储存温度
2-8°C
一般描述
Purified mouse monoclonal antibody gernerated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells. Recognizes the ~95 kDa the β-subunit of insulin receptor.
Recognizes the ~95 kDa the β-subunit of insulin receptor in IM9 cells, placenta, and fetal liver tissue.
This Anti-Insulin Receptor (β-Subunit) Mouse mAb (CT-3) is validated for use in Frozen Sections, Immunoblotting, IP, Paraffin Sections for the detection of Insulin Receptor (β-Subunit).
免疫原
Human
a recombinant protein consisting of the C-terminal 100 amino acids of the β-subunit of human insulin receptor fused to GST
应用
Frozen Sections (1:50-1:100)
Immunoblotting (1:200-1:400)
Immunoprecipitation (not recommended)
Paraffin Sections (1:50-1:100, pressure cooker pre-treatment required)
包装
Please refer to vial label for lot-specific concentration.
外形
In 10 mM PBS, 0.2% BSA, pH 7.4.
制备说明
Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles. Centrifuge the contents of the vial containing the antibody before use to remove any solution that may have accumulated around or under the cap of the vial during shipment or storage.
分析说明
Positive Control
IM-9 lymphocyte cells or placenta or liver tissue
IM-9 lymphocyte cells or placenta or liver tissue
其他说明
Grigorescu, F., et al. 1987. J.Clin. Endocrinol. Metab.64, 549.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.
Rosen, O.M. 1987. Science237, 1452.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.
Morgan, D.O. and Roth, R.A. 1986. Biochemistry25, 1364.
White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.
Ganguly, S., et al. 1985. In Current Topics in Cellular Regulation, Academic Press 27, 83.
Grunberger, G., et al. 1984. Science223, 932.
Hari, J. and Roth, R.A. 1987. J. Biol. Chem.262, 15341.
Rosen, O.M. 1987. Science237, 1452.
Morgan, D.O., et al. 1986. Proc. Natl. Acad. Sci. USA83, 328.
Morgan, D.O. and Roth, R.A. 1986. Biochemistry25, 1364.
White, M.F. and Kahn, C.R. 1986. The Enzymes17, 247.
Ganguly, S., et al. 1985. In Current Topics in Cellular Regulation, Academic Press 27, 83.
Grunberger, G., et al. 1984. Science223, 932.
No cross-reactivity with IGF receptors. Will stain IM-9 lymphocyte cells as well as placenta and liver tissue. This antibody is also useful for tyrosine kinase assays for antibody mediated capture on microwell plates. Antibody should be titrated for optimal results in individual systems.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Toxicity: Standard Handling (A)
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储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Martin B Oleksiewicz et al.
Journal of applied toxicology : JAT, 31(4), 329-341 (2010-10-12)
We compared mitogenicity and intracellular signalling by human insulin and the AspB10 (X-10) human insulin analogue in MCF-7 human mammary adenocarcinoma cells. By flow analysis of phosphorylated histone H3 or cell cycle distributions, insulin and X-10 were mitogenic at physiologically
Jennifer H Law et al.
Cancer research, 68(24), 10238-10246 (2008-12-17)
Drugs that target the insulin-like growth factor-I receptor (IGF-IR) and/or insulin receptor (IR) are currently under investigation for a variety of malignancies including breast cancer. Although we have previously reported that IGF-IR expression in primary breast tumors is common, the
Raju V S Rajala et al.
The Journal of biological chemistry, 277(45), 43319-43326 (2002-09-06)
Recently, we have shown that phosphoinositide 3-kinase (PI3K) in bovine rod outer segment (ROS) is activated in vitro by tyrosine phosphorylation of the C-terminal tail of the insulin receptor (Rajala, R. V. S., and Anderson, R. E. (2001) Invest. Ophthal.
R Klopfleisch et al.
Veterinary and comparative oncology, 8(4), 293-301 (2010-11-11)
Insulin receptor (INSR) or insulin-like growth factor (IGF) signalling is speculated to be involved in mammary tumour development. Expression levels of members of the insulin receptor family (INSR, IGF1R, IGF2R, GHR) and their ligands IGF1and IGF2 were quantified in macro-
Henning Hvid et al.
Journal of applied toxicology : JAT, 31(4), 312-328 (2011-01-25)
Supra-pharmacological doses of the insulin analog X10 (AspB10) increased the incidence of mammary tumors in female Sprague-Dawley rats in chronic toxicity studies, most likely via receptor-mediated mechanisms. However, little is known about the expression of the insulin receptor family in
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