种属反应性
human
assay range
standard curve range: 0.38-6,000 pg/mL
(MIP-1β)
standard curve range: 0.64-10,000 pg/mL
(IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-18, MDC)
standard curve range: 1.3-20,000 pg/mL
(IFNγ, IL-3, IL-17A, RANTES)
standard curve range: 1.6-25,000 pg/mL
(IL-1β, IL-1RA, TNFβ)
standard curve range: 12-200,000 pg/mL
(IL-22, PDGF-AA)
standard curve range: 2.6-40,000 pg/mL
(GM-CSF, IL-10, IP-10, VEGF-A)
standard curve range: 3-50,000 pg/mL
(EGF, Eotaxin, IL-12 (p70), IL-15, MCP-1, MIP-1α)
standard curve range: 32-500,000 pg/mL
(IL-17F)
standard curve range: 4.8-75,000 pg/mL
(G-CSF, IL-1α)
standard curve range: 40-625,000 pg/mL
(IL-17E/IL-25, M-CSF)
standard curve range: 6.4-100,000 pg/mL
(IL-12 (p40), IL-13, MIG, TNFα)
standard curve range: 8-125,000 pg/mL
(IFNα2, MCP-3)
standard curve range: 9.6-150,000 pg/mL
(PDGF-AB/BB)
技术
multiplexing: suitable
检测方法
fluorometric (Luminex® xMAP®)
运输
wet ice
储存温度
2-8°C
一般描述
"Cytokine" is a general term used for a diverse group of soluble proteins and peptides which act as regulators under both normal and pathological conditions to modulate the functional activities of individual cells and tissues. These proteins also mediate direct interactions between cells and regulate processes taking place in the extracellular environment. Cytokines differ from hormones in that they act on a wider spectrum of target cells. The cytokine group of proteins includes lymphokines, interferons, colony stimulating factors and chemokines. Growth factors are involved in the stimulation of target cell survival, proliferation, differentiation with effects on angiogenesis, vasculogenesis, cell migration, apoptosis, wound healing and embryogenesis. Cytokine, chemokine and growth factor research plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process, and disease states such as infectious disease, osteoarthritis, respiratory disease, IBD, sepsis, allergic reactions as well as neurologic, metabolic and cardiovascular disease and even cancer.
MILLIPLEX® offers the broadest selection of analytes across a wide range of disease states and species. Once the analytes of interest have been identified, you can rely on the quality that we build into each kit to produce results you can trust.
Each MILLIPLEX® panel and kit includes:
- Quality controls (QCs) to qualify assay performance
- Comparison of standard (calibrator) and QC lots to a reference lot to ensure lot-to-lot consistency
- Detection antibody cocktails designed to yield consistent analyte profiles within panel.
The MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A SF thus enables you to focus on the therapeutic potential of cytokines and the modulation of cytokine expression. Coupled with the Luminex xMAP platform in a magnetic bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
应用
Analytes available:
EGF, Eotaxin, G-CSF, GM-CSF, IFNα2, IFNγ, IL-1α, IL-1β, IL-1ra, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IP-10, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES, TNFα, TNFβ, VEGF-A.
Application Notes:
- This is an overnight or two-hour incubation assay.
- This assay requires 25 µL of cell culture supernatant per well.
- This kit may be used for the analysis of a selected set of 38 analytes from the Human Cytokine/Chemokine/Growth Factor Panel A in human tissue/cell lysate and culture supernatant samples.
法律信息
免责声明
警示用语:
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
靶器官
Respiratory Tract
储存分类代码
10 - Combustible liquids
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