Legal Information
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Luminex is a registered trademark of Luminex Corp
Application
MILLIPLEX® Qualified assays undergo rigorous assay development, verification, and Quality Control testing to achieve optimal performance. Simultaneously analyze 9 analytes in exosomes isolated from serum, plasma, and tissue culture supernatants.Analytes included: Argonaute-2 (Ago2), Calreticulin (CALR), CD9, CD63, CD81, Flotillin-1 (FLOT-1), GAPDH, Syntenin-1 (Syn-1), and TSG101Note: The Ago2 bead is static and will always be included in the kit format.Assay Characteristics: Refer to kit protocol for assay cross-reactivity, sensitivity, precision, and accuracy.
Disclaimer
For research use only. Not for use in diagnostic procedures.Label License/Sticker for Assay Product:By opening the packaging containing this Assay Product (which contains fluorescently labeled microsphere beads authorized by Luminex Corporation) or using this Assay Product in any manner, you are consenting and agreeing to be bound by the End User Terms and Conditions and the End User License Agreement available at http://support.diasorin.com/end-user-terms-and-conditions/. If you do not agree to all of the terms and conditions, you must promptly return this Assay Product for a full refund prior to using it in any manner.
Features and Benefits
Premixed Bead Format: The panel is provided in a premixed format, where analytes are already combined. This convenience reduces preparation time and minimizes the risk of errors during assay setup, allowing researchers to focus more on their experimental outcomes.Minimal Cross-Reactivity: This specificity ensures accurate and reliable results, reducing the likelihood of false positives and enhancing the validity of the findings.User-Friendly Protocols: The kit comes with clear and concise instructions for assay setup and execution. This minimizes the learning curve for new users, facilitating faster adoption and effective implementation in research settings.Streamlined Workflow: The assay is designed to integrate smoothly into existing laboratory workflows. Researchers can easily incorporate the panel into their studies without significant modifications to their protocols, promoting efficiency and ease of use.Assessment of Functional Components: The ability to assess the functional components of EVs using additional MILLIPLEX® panels for cytokines, chemokines, and growth factors. This comprehensive approach enables researchers to gain insights into the biological activity and signaling pathways associated with exosomes.
General description
Cells release extracellular vesicles (EV), including exosomes, as part of normal physiological processes including removal of waste and regulation of intercellular communication. Furthermore, increased exosome release has been implicated in the progression of various disease states and other abnormalities such as cancer and neurodegeneration. Exosome identification, classification, and differentiation from other extracellular vesicles is often complicated due to the variability in isolation and preparation protocols, exosome biogenesis, as well as exosome source. Commonly associated exosome-enriched markers include tetraspanins CD9, CD63, and CD81, cargo sorting-associated markers TSG101 and Flotillin-1, and biogenesis marker syntenin-1. Other markers, including Ago2, GAPDH, and Calreticulin, may or may not be found in exosome-enriched samples, but this can vary depending on source, isolation method, and/or EV biogenesis mechanism.
