Anti-Desmin (Ab-1) Mouse mAb (DE-B-5)

Purified mouse monoclonal antibody generated by immunizing mice with the specified immunogent and fusing splenocytes with PAI mouse myeloma cells (see application references). Recognizes the ~53 kDa Desmin protein.

Human

Frozen Sections (1-5 µg/ml, see application references)

Immunoblotting (1-5 µg/ml, see application references)

Immunocytochemistry (1-5 µg/ml, see application references)

Paraffin Sections (1-10 µg/ml, heat pre-treatment required, see application references)

Lyophilized from 20 mM NH₄HCO₃, 100 µg BSA.

We recommend resuspending the lyophilized antibody with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Avoid freeze/thaw cycles. Antibody should be titrated for optimal results in individual systems.

Negative Control
Epithelial cells or tissue

Positive Control
Muscle cells or tissue

Debus, E., et al. 1983. EMBO J.2, 2305.
Geisler, N., et al. 1982. Cell30, 277.
Lazarides, E. 1982. Ann. Rev. Biochem.51, 219.
Osborn, M. and Weber, K., 1982. Cell31, 303.

This antibody stains intermediate filaments found in skeletal, visceral and vascular smooth muscle cells, as well as those found in certain established cell lines such as the human rhabdomyosarcoma line (see application references). Desmin is also found in rhabdomyosarcoma biopsies, and is positively stained in smaller, round cells, which are difficult to judge histologically as rhabdomyosarcoma. Does not recognize other classes of intermediate filament proteins (see application references). Antibody should be titrated for optimal results in individual systems.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Toxicity: Standard Handling (A)