Bone Sialoprotein II is one of the major noncollagenous proteins in the extracellular matrix of bone. It is a phosphorylated glycoprotein with an approximate molecular weight of 70 kDa. The protein has been found in osteoblasts and osteocytes. Diseases concerning bone turnover, pathological bone alterations, as well as the rapid increase of osteoporosis make it necessary to establish new markers. Bone Sialoprotein is discussed as a potential serum marker for monitoring bone remodeling.

Recombinant and native human bone sialoprotein II (BSPII). Does not react with osteopontin (BSPI) or osteonectin.

Bone Sialoprotein isolated from human bones.

Epitope: C-terminus

Research Category
Cell Structure

Research Sub Category
Osteobiology

This Anti-Bone Sialoprotein II Antibody, C-terminus, clone ID1.2 is validated for use in ELISA, RIA, WB, IC, IH(P) for the detection of Bone Sialoprotein II.

Western blot: (1μg/mL)

Immunohistochemistry on paraffin embedded tissue sections: (1μg/mL)

Immunocytochemistry: (1:100)

ELISA: (1μg/mL)

RIA

Optimal working dilutions must be determined by the end user.

Replaces: AB1855

Format: Purified

Purified immunoglobulin from culture supernatant. Lyophilized from 50 mM TRIS, pH7.4.

Maintain lyophilized material at 2-8°C. Reconstitute with 100μL sterile distilled water and store at -20°C in undiluted aliquots up to 12 months. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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