biological source
rat
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
187.1, monoclonal
species reactivity
mouse
technique(s)
ELISA: suitable, flow cytometry: suitable
isotype
IgG1κ
shipped in
wet ice
Quality Level
Gene Information
mouse ... Igk(243469)
General description
25 kDa Calculated
Antibody molecules typically comprise two immunoglobulin light chains covalently bound to a pair of heavy chains. Immunoglobulin light chains occur in two types, designated by the Greek letters kappa and lambda. Kappa and lambda light chains are approximately 250 amino acids in length with an average mass of about 25 kDa. The ratio of kappa to lambda found in the immunoglobulin population varies by species.
Immunogen
Epitope: Unknown
Mouse IgG2b, κ secreted by MPC-11 plasmacytoma
Application
Anti-mouse Immunoglobulin kappa light chain Antibody, clone 187.1, Rat is an antibody against mouse Immunoglobulin kappa light chain for use in ELISA, FC.
Flow Cytometry Analysis: A previous lot was used by an independent laboratory in FC. (Beck, K., et al. (2009). J. Exp. Med.206:2271-2284.)
Research Category
Secondary & Control Antibodies
Secondary & Control Antibodies
Research Sub Category
Fragment Specific Secondary Antibodies
Fragment Specific Secondary Antibodies
Biochem/physiol Actions
This antibody specifically recongizes the kappa light chain of mouse antibodies.
Physical form
Format: Purified
Protein G
Purified rat monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by ELISA Analysis in a titer.
ELISA Analysis: This antibody was successfully used in a titer ELISA. Specificity of detection for mouse kappa light chain peptides is displayed below.
ELISA Analysis: This antibody was successfully used in a titer ELISA. Specificity of detection for mouse kappa light chain peptides is displayed below.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10-13 - German Storage Class 10 to 13
法规信息
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Teresa Maria Carusone et al.
Cell death & disease, 11(5), 324-324 (2020-05-10)
The activity of human paraoxonase 2 (PON2) is rapidly reduced in cells incubated with the bacterial quorormone 3-Oxo-dodecanoyl Homoserine Lactone (3OC12HSL), an observation that led to hypothesize a fast PON2 post-translational modification (PTM). Recently, we detected a 3OC12HSL-induced PTM in
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