biological source
mouse
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
47-9, monoclonal
species reactivity
human, pig, sheep, bovine
should not react with
rat, mouse
manufacturer/tradename
Chemicon®
isotype
IgG1
suitability
not suitable for Western blot, not suitable for immunoprecipitation
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... INSR(3643)
Immunogen
Epitope: alpha subunit, azide free
IM-9 lymphocytes followed by purified insulin receptor.
Application
Anti-Insulin Receptor Antibody, α subunit, azide free, clone 47-9 detects level of Insulin Receptor & has been published & validated.
Completely (>90%) Inhibits Insulin Binding: use antibody at 10nM
Antagonist: Blocks insulin action
Does not work in immunoprecipitation {Soos, 1986}.
Optimal working dilutions must be determined by end user.
Antagonist: Blocks insulin action
Does not work in immunoprecipitation {Soos, 1986}.
Optimal working dilutions must be determined by end user.
Research Category
Signaling
Signaling
Research Sub Category
Insulin/Energy Signaling
Insulin/Energy Signaling
Biochem/physiol Actions
Recognizes a protein of 135kDa, identified as the alpha-subunit of insulin receptor (IR). Its epitope localizes in exon 7/8 and shows no cross-reaction with IGF-receptors {Soos, 1986}. Antibody superbly inhibits (>90%) insulin binding and BLOCKS insulin action. IR is a heterotetrameric membrane glycoprotein consisting of disulfide-linked subunits in a beta-a-a-beta configuration. The beta-subunit (95kDa) possesses a single transmembrane domain, whereas the alpha-subunit is completely extracellular. The intracellular portion of the beta-subunit is a tyrosine-specific protein kinase with sequence homology to other such kinases. Activation of kinase is essential in signaling pathway whereby insulin regulates intracellular metabolism.
Physical form
Format: Purified
Protein G Purified
Protein G Purified mouse immunoglobulin in 10mM PBS, pH 7.4 contain no preservatives.
Preparation Note
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
3T3 cells expressing transfected human insulin receptor
3T3 cells expressing transfected human insulin receptor
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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The Garcia effect, a solid learning paradigm, was used to investigate the molecular and behavioral effects induced by different lengths of fasting on the cognitive functions in the pond snail Lymnaea stagnalis, a valid model system. Three experimental groups were
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