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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
G3, monoclonal
Application:
flow cytometry
immunohistochemistry
western blot
immunohistochemistry
western blot
Species reactivity:
human
Citations:
88
Technique(s):
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
immunohistochemistry: suitable
western blot: suitable
Uniprot accession no.:
产品名称
抗类胰蛋白酶抗体,肥大细胞,克隆G3, clone G3, Chemicon®, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
clone
G3, monoclonal
species reactivity
human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable
immunohistochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Quality Level
相关类别
Analysis Note
对照
肥大细胞,嗜碱粒细胞
人胎儿皮肤裂解物
肥大细胞,嗜碱粒细胞
人胎儿皮肤裂解物
已通过蛋白质印迹法在人胎儿皮肤裂解物中进行了评估。
蛋白质印迹分析:0.5 µg/mL的该抗体在10 µg人胎儿皮肤裂解中检测到类胰蛋白酶。
蛋白质印迹分析:0.5 µg/mL的该抗体在10 µg人胎儿皮肤裂解中检测到类胰蛋白酶。
Application
流式细胞术分析:该抗体的先前批次已使用偶联的二抗在FACS中的固定细胞上使用。(Sperr,W.,et al.,2001)。
最佳工作稀释度必须由最终用户确定。
最佳工作稀释度必须由最终用户确定。
研究子类别
炎症 & 自身免疫机制
炎症 & 自身免疫机制
研究类别
炎症 & 免疫学
炎症 & 免疫学
该抗类胰蛋白酶抗体,肥大细胞,克隆G3经验证可用于FC、IH、WB检测类胰蛋白酶。
Biochem/physiol Actions
识别肥大细胞类胰蛋白酶。对嗜碱性粒细胞有反应性,但程度较轻。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
31kda
类胰蛋白酶是肥大细胞中含有的最丰富的分泌颗粒衍生的丝氨酸蛋白酶。类胰蛋白酶最近被用作肥大细胞活化的标志物,并与过敏反应有关。类胰蛋白酶可作为成纤维细胞系的促分裂原。 过敏反应和类过敏反应均会升高血清类胰蛋白酶水平,但阴性试验并不排除过敏反应。
Other Notes
浓度:请参考批次特异性浓缩物的分析证书。
Physical form
形式:纯化
纯化的小鼠单克隆IgG1κ,溶于含0.1 M Tris-甘氨酸(pH值7.4,150 mM NaCl)和0.05%叠氮化钠的缓冲液中。
(具体缓冲液配方见产品数据表)
(具体缓冲液配方见产品数据表)
纯化的蛋白G
Preparation Note
在产品数据表上列出的持续时间内保持在2–8°C。(贮藏条件见产品数据表)
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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