The nucleoli small basophilic, spherical, non-membrane enclosed, highly conserved, structures that are localized within the nucleus. Usually, they are localized in the central nuclear region or close to the nuclear membrane. They are formed around nucleolus organizer regions (NORs) consisting of ribosomal DNA (rDNA). They are structurally organized into the fibrillar center, the dense fibrillar component, and the granular component region. Clone 125-10 detects human nucleoli by immunocytochemistry.

Raji cell nuclei.

Quality Control Testing

Evaluated by Immunocytochemistry in A431 cells.

Immunocytochemistry Analysis: A 1:1,000 dilution of this antibody detected Nucleoli in A431 cells.

Tested Applications

Immunocytochemistry Analysis: A representative lot detected Nucleoli in Immunocytochemistry applications (Endo, A., et al. (2009). J Biol Chem. 284(41):27918-27923; Endo, A., et al. (2009). J Cell Sci. 122(Pt 5):678-86).

Immunofluorescence Analysis: A representative lot detected Nucleoli in Immunofluorescence applications (Clements, D., et al. (2007). Am J Physiol Lung Cell Mol Physiol. 292(1):L258-66; Vallee, D., et al. (2004). J Med Genet. 41(10):778-83; Ohno, M., et al. (2002). Chromosoma. 111(3):201-13).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-Nucleoli, clone 125-10, Cat. No. MAB1277-I, is a mouse monoclonal antibody that detects nucleoli and is tested for use in Immunocytochemistry, Immunofluorescence, and Immunocytochemistry.

Clone 125-10 is a mouse monoclonal antibody that specifically detects nucleoli in human cells.

Purified mouse monoclonal antibody IgG1 in PBS without preservatives.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.