产品名称
Anti-mCherry Antibody, clone 1C51, clone 1C51, from mouse
biological source
mouse
conjugate
unconjugated
antibody form
purified antibody
antibody product type
primary antibodies
clone
1C51, monoclonal
species reactivity (predicted by homology)
all
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG2aκ
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Analysis Note
Evaluated by Western Blotting in HEK293 cells transfected with pFin-EF1-mCherry.
Western Blotting Analysis: 1 µg/mL of this antibody detected mCherry in 10 µg of lysate from HEK293 cells transfected with pFin-EF1-mCherry.
Western Blotting Analysis: 1 µg/mL of this antibody detected mCherry in 10 µg of lysate from HEK293 cells transfected with pFin-EF1-mCherry.
Application
Anti-mCherry Antibody, clone IC51, Cat. No. MAB131873 detects mCherry protein and is tested for use in Immunocytochemistry and Western Blotting.
Western Blotting Analysis: A representative lot detected mCherry in Western Blotting applications (Krajcovic, M., et. al. (2013). Mol Biol Cell. 24(23):3736-45).
Western Blotting Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vs HEK293 untransfected cells (Courtesy of Dr Gerry Shaw).
Immunocytochemistry Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry (Courtesy of Dr Gerry Shaw).
Western Blotting Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry vs HEK293 untransfected cells (Courtesy of Dr Gerry Shaw).
Immunocytochemistry Analysis: A representative lot detected mCherry in HEK293 cells transfected with pFin-EF1-mCherry (Courtesy of Dr Gerry Shaw).
Biochem/physiol Actions
Clone 1C51 detects mCherry protein in multiple species.
General description
mCherry, red fluorescent protein, is derived from DsRed, a red fluorescent protein from disc corals of Discosoma species. It is monomeric form that has been engineered with multiple cycles of mutation, directed modification and evolutionary selection. It exhibits excitation maximum at 587 nm and an emission maximum at 610 nm with a fluorescent quantum yield of 0.22. mCherry displays high photostability and resistant to photobleaching. It also displays improved brightness and extremely rapid maturation rate. Anti-mCherry, clone1C51 is an affinity purified antibody that recognizes mCherry in HEK293 cells transfected with pFin-EF1-mCherry. (Ref.: Schaner, NC et al. (2004). Nat. Biotechnol. 22(12):1567-72).
~32 kDa Observed; 26.72 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
His-tagged full length recombinant mCherry.
Other Notes
Concentration: Please refer to lot specific datasheet.
Physical form
Format: Purified
Purified mouse monoclonal antibody IgG2a in buffer containing PBS with 0.03% sodium azide with 50% glycerol.
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存储类别
10 - Combustible liquids
wgk
WGK 2
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