MAB16200-AF488
Anti-MGMT Antibody, clone MT 3.1, Alexa Fluor™ 488 Conjugate
clone MT3.1, from mouse, ALEXA FLUOR™ 488
别名:
Methylated-DNA-protein-cysteine methyltransferase, 6-O-methylguanine-DNA methyltransferase, GATase, MGMT, O-6-methylguanine-DNA-alkyltransferase
biological source
mouse
conjugate
ALEXA FLUOR™ 488
antibody form
purified antibody
antibody product type
primary antibodies
clone
MT3.1, monoclonal
species reactivity
human
technique(s)
flow cytometry: suitable, immunocytochemistry: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Quality Level
Gene Information
human ... MGMT(4255)
General description
Methylated-DNA-protein-cysteine methyltransferase (EC 2.1.1.63; UniProt P16455; also known as 6-O-methylguanine-DNA methyltransferase, GATase, MGMT, O-6-methylguanine-DNA-alkyltransferase) is encoded by the MGMT gene (Gene ID 4255) in human. MGMT is a DNA repair enzyme that removes alkyl groups from O6-guanine and plays a fundamental role in maintaining genomic integrity. MGMT converts the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine, thereby prevents mismatch and errors during DNA replication and transcription. MGMT expression level correlates with cellular resistance to alkylnitrosoureas, compounds that induce O6-alkylguanine lesions. MGMT transcription is negatively regulated via promoter methylation, MGMT promoter methylation level is reported to correclate with the survival rate of glioblastoma patients receiving treatment with alkylating agents.
21.65 kDa calculated. ~25 kDa reported (Brent, T.P., et al. (1990). Cancer Res. 50(1):58-61).
Application
Anti-MGMT Antibody, clone MT 3.1, Alexa Fluor™ 488 Conjugate, Cat. No. MAB16200-AF488, is a highly specific mouse monoclonal antibody that targets MGMT and has been tested in Flow Cytometry and Immunocytochemistry.
Flow Cytometry Analysis: 0.2 µL from a representative lot detected MGMT in one million MCF-7 cells.
This monoclonal antibody is also available in unconjugated and Alexa Fluor™ 647-conjugated forms (Cat. No. MAB16200 and MAB16200-AF647) for flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.
This monoclonal antibody is also available in unconjugated and Alexa Fluor™ 647-conjugated forms (Cat. No. MAB16200 and MAB16200-AF647) for flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.
Biochem/physiol Actions
Clone MT 3.1 detected p53 expression-dependent MGMT downregulation in IMR90 cells (Harris, L.C., et al. (1996). Cancer Res. 56(9):2029-2032).
Physical form
Purified mouse IgG1 Alexa Fluor™ 488 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
Analysis Note
Evaluated by Immunocytochemistry in MCF-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this Alexa Fluor™ 488-conjugated antibody detected MGMT in MCF-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this Alexa Fluor™ 488-conjugated antibody detected MGMT in MCF-7 cells.
Other Notes
Concentration: Please refer to lot specific datasheet.
Legal Information
ALEXA FLUOR is a trademark of Life Technologies
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存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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