生物来源
mouse
质量水平
偶联物
ALEXA FLUOR™ 488
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
MT3.1, monoclonal
种属反应性
human
技术
flow cytometry: suitable
immunocytochemistry: suitable
同位素/亚型
IgG1
NCBI登记号
UniProt登记号
运输
ambient
靶向翻译后修饰
unmodified
基因信息
human ... MGMT(4255)
一般描述
Methylated-DNA-protein-cysteine methyltransferase (EC 2.1.1.63; UniProt P16455; also known as 6-O-methylguanine-DNA methyltransferase, GATase, MGMT, O-6-methylguanine-DNA-alkyltransferase) is encoded by the MGMT gene (Gene ID 4255) in human. MGMT is a DNA repair enzyme that removes alkyl groups from O6-guanine and plays a fundamental role in maintaining genomic integrity. MGMT converts the naturally occurring mutagenic DNA lesion O6-methylguanine back to guanine, thereby prevents mismatch and errors during DNA replication and transcription. MGMT expression level correlates with cellular resistance to alkylnitrosoureas, compounds that induce O6-alkylguanine lesions. MGMT transcription is negatively regulated via promoter methylation, MGMT promoter methylation level is reported to correclate with the survival rate of glioblastoma patients receiving treatment with alkylating agents.
21.65 kDa calculated. ~25 kDa reported (Brent, T.P., et al. (1990). Cancer Res. 50(1):58-61).
应用
Anti-MGMT Antibody, clone MT 3.1, Alexa Fluor™ 488 Conjugate, Cat. No. MAB16200-AF488, is a highly specific mouse monoclonal antibody that targets MGMT and has been tested in Flow Cytometry and Immunocytochemistry.
Flow Cytometry Analysis: 0.2 µL from a representative lot detected MGMT in one million MCF-7 cells.
This monoclonal antibody is also available in unconjugated and Alexa Fluor™ 647-conjugated forms (Cat. No. MAB16200 and MAB16200-AF647) for flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.
This monoclonal antibody is also available in unconjugated and Alexa Fluor™ 647-conjugated forms (Cat. No. MAB16200 and MAB16200-AF647) for flow cytometry, immunocytochemistry, immunohistochemistry, immunoprecipitation, and Western blotting applications.
生化/生理作用
Clone MT 3.1 detected p53 expression-dependent MGMT downregulation in IMR90 cells (Harris, L.C., et al. (1996). Cancer Res. 56(9):2029-2032).
外形
Purified mouse IgG1 Alexa Fluor™ 488 conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
分析说明
Evaluated by Immunocytochemistry in MCF-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this Alexa Fluor™ 488-conjugated antibody detected MGMT in MCF-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution of this Alexa Fluor™ 488-conjugated antibody detected MGMT in MCF-7 cells.
其他说明
Concentration: Please refer to lot specific datasheet.
法律信息
ALEXA FLUOR is a trademark of Life Technologies
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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