生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
ascites fluid
抗体产品类型
primary antibodies
克隆
23IIC3, monoclonal
种属反应性
monkey, human, rat
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
靶向翻译后修饰
unmodified
基因信息
human ... COL4A2(1284)
一般描述
170 kDa
免疫原
人羊膜IV型胶原蛋白
应用
使用经验证可用于ELISA、IH(P)& WB的该抗IV型胶原蛋白α 2链抗体(克隆23IIC3)检测IIV型胶原蛋白α 2链。
免疫组化:丙酮固定、冷冻组织建议采用1:500。 在1%胰蛋白酶处理(20分钟,室温)后,在福尔马林固定的石蜡切片(1:100)上有微弱的反应性。 或者,4% PFA 固定、HEIR处理的组织在1:200稀释度下对猴有效(Qin, 2003)。
蛋白质印迹:1:500, 170 kDa.
1:200 的ELISA
最佳工作稀释度必须由最终用户进行确定。
蛋白质印迹:1:500, 170 kDa.
1:200 的ELISA
最佳工作稀释度必须由最终用户进行确定。
研究子类别
ECM 蛋白
ECM 蛋白
研究类别
细胞结构
细胞结构
生化/生理作用
识别人IV型胶原蛋白α-2链。
外形
未纯化
液体。
制备说明
自收到之日起在-20°C可稳定保存1年。避免反复冻融。为了最大程度地回收产品,在融化后和取下盖子之前,将原始小瓶进行离心。
分析说明
对照
阳性对照:肾脏、肌肉、肌腱、脾脏组织
阴性对照:神经元/神经胶质
阳性对照:肾脏、肌肉、肌腱、脾脏组织
阴性对照:神经元/神经胶质
其他说明
浓度:请参考批次特异性浓缩物的检验报告。
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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储存分类代码
10 - Combustible liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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