生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified antibody
抗体产品类型
primary antibodies
克隆
9F8, monoclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
ELISA: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable
同位素/亚型
IgG1κ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... TNC(3371)
免疫原
Epitope: type III repeats 1-3
Purified human tenascin
应用
Immunohistochemistry at 2 μg/mL on tissues fixed with cold acetone
Immunocytochemistry at 2 μg/mL on U251 cells
Immunoblotting (Western) at 2 μg/mL to detect whole tenascin under reducing conditions
ELISA: 2 x 10-10 M endpoint dilution
Affinity constant Kd= 6.37 x 10-9
Final working dilutions must be determined by end user.
Immunocytochemistry at 2 μg/mL on U251 cells
Immunoblotting (Western) at 2 μg/mL to detect whole tenascin under reducing conditions
ELISA: 2 x 10-10 M endpoint dilution
Affinity constant Kd= 6.37 x 10-9
Final working dilutions must be determined by end user.
This Anti-Tenascin Antibody, type III repeats 1-3, clone 9F8 is validated for use in ELISA, WB, IC, IH for the detection of Tenascin.
生化/生理作用
Binds to a recombinant fragment of human tenascin containing the fibronectin type III repeats, numbers 1-3.
外形
Format: Purified
Liquid in 0.02 M phosphate, 0.25 M Sodium Chloride pH 7.6, with 0.1% Sodium Azide.
制备说明
Store at 2 and 8°C for up to 6 months.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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储存分类代码
10 - Combustible liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
James Ozanne et al.
Frontiers in immunology, 14, 1275361-1275361 (2023-12-11)
Serum biomarkers are the gold standard in non-invasive disease diagnosis and have tremendous potential as prognostic and theranostic tools for patient stratification. Circulating levels of extracellular matrix molecules are gaining traction as an easily accessible means to assess tissue pathology.
Investigating Chemokine-Matrix Networks in Breast Cancer: Tenascin-C Sets the Tone for CCL2.
Gschwandtner, et al.
International Journal of Molecular Sciences, 24 (2023)
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