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About This Item

UNSPSC代码:
12352203
eCl@ss:
32160702
NACRES:
NA.41
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Product Name

Anti-Integrin β1 Antibody, clone P4G11, azide free, clone P4G11, Chemicon®, from mouse

生物来源

mouse

质量水平

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

P4G11, monoclonal

种属反应性

monkey, human, rabbit

制造商/商品名称

Chemicon®

技术

ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable

同位素/亚型

IgG1

UniProt登记号

运输

wet ice

靶向翻译后修饰

unmodified

基因信息

human ... ITGB1(3688)

免疫原

Human IL-1beta activated endothelial cells

应用

Immunohistochemistry: for use on acetone or mild paraformaldehyde fixed tissue (2-2.5% PFA, short time) frozen and 4% PFA paraffin embedded {Qin et al 2003 1:500, citrate HEIR treated}

Ca++ required for binding. Ca++ must be used in blocking, washing, and antibody incubation buffers.

Traditional formalin fixation is not recommended.

Immunocytochemistry: 2 ug/mL (for use on numerous cell lines as long as Ca2+ is present)

Immunoprecipitation: 5 μg/400 μL lysate (0.5% triton PBS plus Ca++)

FACS: 2-5μg per 10e6 cells, in the presence of Ca++.

Stimulation of cells: 2-10 μg/ml per 10e6 cells. Binding buffer must contain 1mM Ca++.

ELISA

Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Research Sub Category
Integrins
This Anti-Integrin β1 Antibody, clone P4G11, azide free is validated for use in ELISA, FC, IP, FUNC, FUNC, IC, IH for the detection of Integrin β1.

生化/生理作用

Human integrin beta1. The epitope recognized by MAB1951Z is Ca2+ dependent.

外形

Format: Purified
Sterile-filtered protein A purified immunoglobulin in 0.01M PBS, pH 7.1, containing no preservatives.

制备说明

Maintain between 2 and 8°C for up to 6 months from date of receipt. Avoid freeze thaws.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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