产品名称
Anti-Integrin α5β1 Antibody, clone HA5, clone HA5, Chemicon®, from mouse
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
HA5, monoclonal
species reactivity
pig, human
manufacturer/tradename
Chemicon®
technique(s)
flow cytometry: suitable, immunoprecipitation (IP): suitable
isotype
IgG2bκ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ITGA5(3678)
Application
Anti-Integrin α5β1 Antibody, clone HA5 detects level of Integrin α5β1 & has been published & validated for use in FC & IP.
Immunoprecipitation of VLA-5 integrins from lysate of 10E6 cell equivalent requires 2 μg of MAB1999.
Flow cytometry: 10 μg/mL
Does not detect VLA-5 by Western blot.
MAB1999 does not affect VLA-5 mediated cell adhesion to fibronectin.
Optimal working dilutions must be determined by end user.
Flow cytometry: 10 μg/mL
Does not detect VLA-5 by Western blot.
MAB1999 does not affect VLA-5 mediated cell adhesion to fibronectin.
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Reacts with the fibronectin receptor VLA-5 (alpha-5beta-1) integrin complex.
Physical form
Format: Purified
Purified immunoglobulin from Protein A chromatography. Liquid in 0.02 M PB pH 7.6, 0.25 M NaCl, 0.1% sodium azide.
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
存储类别
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Yuya Sato et al.
The Journal of biological chemistry, 284(18), 11873-11881 (2009-03-12)
Recently we reported that N-glycans on the beta-propeller domain of the integrin alpha5 subunit (S-3,4,5) are essential for alpha5beta1 heterodimerization, expression, and cell adhesion. Herein to further investigate which N-glycosylation site is the most important for the biological function and
Ingrid Burvenich et al.
Molecular cancer therapeutics, 7(12), 3771-3779 (2008-12-17)
This study identifies and characterizes the antigen recognized by monoclonal antibody (mAb) 14C5. We compared the expression of antigen 14C5 with the expression of eight integrin subunits (alpha1, alpha2, alpha3, alphav, beta1, beta2, beta3, and beta4) and three integrin heterodimers
U Ruth Michaelis et al.
Circulation research, 112(6), 924-934 (2013-01-31)
Polarity proteins are involved in the apico-basal orientation of epithelial cells, but relatively little is known regarding their function in mesenchymal cells. We hypothesized that polarity proteins also contribute to endothelial processes like angiogenesis. Screening of endothelial cells revealed high
全球贸易项目编号
| 货号 | GTIN |
|---|---|
| MAB1999 | 04053252315848 |