生物来源
mouse
质量水平
抗体形式
culture supernatant
抗体产品类型
primary antibodies
克隆
N29, monoclonal
种属反应性
human
制造商/商品名称
Chemicon®
技术
cell culture | mammalian: suitable
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型
IgGκ
NCBI登记号
UniProt登记号
运输
wet ice
靶向翻译后修饰
unmodified
基因信息
human ... ITGB1(3688)
相关类别
一般描述
88 kDa
免疫原
Jurkat T-leukemic cell line
应用
Immunohistochemistry (Paraffin) Analysis: A 1:50-2,000 dilution from a representative lot detected Integrin β1 in the membrane of tubule epithelial cells and cells of the glomeruli in human kidney tissue, membrane of alveolar cells, endothelial cells, and bronchiole epithelial cells of human lung tissue.
Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.
Flow Cytometry: A representative lot of this antibody clone was used in flow cytometry.
Functional Activity Assay: A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).
Optimal working dilutions must be determined by end user.
Immunoprecipitation: A representative lot of this antibody clone was used in immunoprecipitation.
Immunohistochemistry: A representative lot of this antibody clone was used in immunohistochemistry on frozen sections.
Flow Cytometry: A representative lot of this antibody clone was used in flow cytometry.
Functional Activity Assay: A representative lot of this antibody clone was used to stimulate adhesion of cells to extracellular matrix proteins (Wilkins, J.A. et al., 1996).
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin β1 Antibody, clone N29 (Azide Free) is validated for use in Flow Cytometry and Immunohistochemistry and Immunoprecipitation and Affects Function and Cell Culture and Western Blotting for the detection of Integrin β1.
生化/生理作用
Reacts with the human integrin beta1 subunit. Specificity verified by preclearing of beta1in immunoprecipitation, flow cytometry on transfectant cells displaying human beta1 integrin, and reactivity with purified beta1. Recognizes an epitope cluster distinct from MAB2250 and MAB2251(Wilkins et al., 1996).
外形
Protein A purified
Format: Purified
Purified cultured supernatant in PBS without preservatives.
制备说明
Store at -20°C in undiluted aliquots.
Avoid repeated freeze-thaw cycles.
Avoid repeated freeze-thaw cycles.
分析说明
Western Blot Analysis: 0.5 µg/mL of the antibody detected Integrin β1 in 10 µg of U251 cell lysate.
Control
Tonsil, human skin, human kidney tissue
U251 cell lysate
Tonsil, human skin, human kidney tissue
U251 cell lysate
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 2
闪点(°F)
Not applicable
闪点(°C)
Not applicable
Rania Hadjisavva et al.
Cell reports, 40(3), 111091-111091 (2022-07-21)
Cadherins and integrins are intrinsically linked through the actin cytoskeleton and are largely responsible for the mechanical integrity and organization of tissues. We show that cadherin clustering stimulates and spatially guides integrin activation. Adherens junction (AJ)-associated integrin activation depends on
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