MAB331
Anti-SNAP-25 Antibody, clone SP14
ascites fluid, clone SP14, Chemicon®
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UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Clone:
SP14, monoclonal
Species reactivity:
pig, human, hamster, rat
Application:
ELISA, IHC, WB
Citations:
35
biological source
mouse
antibody form
ascites fluid
clone
SP14, monoclonal
species reactivity
pig, human, hamster, rat
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunohistochemistry: suitable (paraffin), western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... SNAP25(6616)
Immunogen
Crude synaptic immunoprecipitate (Human)
Application
Detect SNAP-25 using this Anti-SNAP-25 Antibody, clone SP14 validated for use in ELISA, WB, IH(P).
Immunohistochemistry at 1:500-1:5,000
Formalin fixed (<4 weeks), paraffin embedded
* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.
Frozen
Western blotting at 1:100-1:1,000
ELISA at 1:500
It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.
Optimal working dilutions must be determined by the end user.
Formalin fixed (<4 weeks), paraffin embedded
* It is suggested that you use an IgG specific second antibody to minimize possible non-specific staining.
Frozen
Western blotting at 1:100-1:1,000
ELISA at 1:500
It is reported that clone SP14 is capable of detecting SDS-resistant SNARE complexes {J. Cell Sci 2004 117(6):955-966}.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Synapse & Synaptic Biology
Synapse & Synaptic Biology
Biochem/physiol Actions
SNAP-25 (26-27 kDa). MAB331 is reactive with fusion protein from COS cells, but not with fusion protein from bacterial systems.
Physical form
Liquid. Contains no preservative.
Preparation Note
Maintain frozen at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Matthew J Van Hook et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 37(17), 4618-4634 (2017-04-02)
G-protein βγ subunits (Gβγ) interact with presynaptic proteins and regulate neurotransmitter release downstream of Ca2+ influx. To accomplish their roles in sensory signaling, photoreceptor synapses use specialized presynaptic proteins that support neurotransmission at active zone structures known as ribbons. While
Bronchoepithelial expression of CXCR1 and CXCR2 does not facilitate transepithelial migration of neutrophils.
Christian Schulz,Benjamin Stoelcker,Brigitte Ruhland,Nico Jentsch,Andreas Steege
Respiration; International Review of Thoracic Diseases null
High sensitivity of mouse neuronal cells to tetanus toxin requires a GPI-anchored protein.
Munro, P; Kojima, H; Dupont, JL; Bossu, JL; Poulain, B; Boquet, P
Biochemical and biophysical research communications null
Most peptide-containing sensory neurons lack proteins for exocytotic release and vesicular transport of glutamate.
Morris, JL; Konig, P; Shimizu, T; Jobling, P; Gibbins, IL
The Journal of Comparative Neurology null
Toshihiko Shirafuji et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 38(2), 278-290 (2017-11-24)
Protein kinase Cγ (PKCγ) knock-out (KO) animals exhibit symptoms of Parkinson's disease (PD), including dopaminergic neuronal loss in the substantia nigra. However, the PKCγ substrates responsible for the survival of dopaminergic neurons in vivo have not yet been elucidated. Previously
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