biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
6C1, monoclonal
species reactivity
mouse, sheep, rat, human, chicken, Xenopus
manufacturer/tradename
Chemicon®
technique(s)
ELISA: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable
isotype
IgM
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Quality Level
Gene Information
human ... DAG1(1605)
Immunogen
Epitope: aa572-604 of human sequence
Synthetic peptide corresponding to a.a. 572-604 of human cranin.
Application
Immunohistochemistry on tissue fixed briefly with 0.5% glutaraldehyde, 2% PLP fixation, methanol or acetone. Prolonged fixation must be avoided. Traditional formalin is not recommended. Suggested working dilution 1:1-1:50.
Immunocytochemistry on neuron, astrocyte, smooth muscle, fibroblast and epithelial cell lines.
ELISA
Not suggested for use in Western blot.
Optimal working dilutions must be determined by end user.
Immunocytochemistry on neuron, astrocyte, smooth muscle, fibroblast and epithelial cell lines.
ELISA
Not suggested for use in Western blot.
Optimal working dilutions must be determined by end user.
Research Category
Cell Structure
Cell Structure
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
ECM Proteins
Cytoskeleton
ECM Proteins
This Anti-Cranin Antibody, aa572-604 of human sequence, clone 6C1 is validated for use in ELISA, IC, IH for the detection of Cranin.
Biochem/physiol Actions
Cranin (dystroglycan), extracellular site, near the C-terminus of the alpha-subunit. The expression and localization of cranin varies considerably according to the cells physiologic state and differentiaion state. MAB371 recognizes the same peptide region as MAB373 but a different epitope.
Physical form
Ascites Liquid.
Preparation Note
Maintain at -20°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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存储类别
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
X Li et al.
The Journal of cell biology, 153(4), 811-822 (2001-05-16)
Fibroblast growth factors and receptors are intimately connected to the extracellular matrix by their affinity to heparan sulfate proteoglycans. They mediate multiple processes during embryonic development and adult life. In this study, embryonic stem cell-derived embryoid bodies were used to
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