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Merck
CN

MAB4308

Anti-VIN-IS-56 Antibody, clone VIN-IS-56

clone VIN-IS-56, Chemicon®, from mouse

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eCl@ss:
32160702
UNSPSC Code:
12352203

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biological source

mouse

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

VIN-IS-56, monoclonal

species reactivity (predicted by homology)

mammals

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable, immunocytochemistry: suitable, immunohistochemistry: suitable

input

sample type neural stem cell(s)

isotype

IgM

shipped in

wet ice

target post-translational modification

unmodified

General description

Almost all melanomas, astrocytomas,a proportion of sarcomas, a small number of carcinomas, some nevi, as well as normal melanocytes express GD3 antigen.
GD3 is one of the most important markers for malignant melanoma. Antibodies to the GD3 ganglioside can induce partial remission of tumor growth in animals as well as in humans via enhancement of cytotoxic and proliferative response of lymphocytes.
Anti-GD3 antibodies have been found in association with specific forms of Guillain-Barre syndrome. In vivo studies of isolated anti-GM1 and GD3 antibodies indicate the antibodies can interfere with motor neuron function.

Immunogen

Human Embryonal carcinoma cells

Application

Anti-VIN-IS-56 Antibody, clone VIN-IS-56 detects level of VIN-IS-56 & has been published & validated for use in FC, IC, IH.
Immunohistochemistry & Immunocytochemistry:

Suggested fixatives: Paraformaldehyde is recommended, NOT methanol or acetone (the glycolipid antigen is extracted by polar lipid solvents).

Recommended antibody dilution: 10-20 μg/ml

Dilution Buffer: PBS + 4% FCS

Expected staining pattern: cell surface staining



FACS Analysis:

Suggested dilution/number of cells: 10-20 μg/ml for 1 x 105 cells

Fixation/Permeabilization used: Live cells or fixed in 4% Paraformaldehyde

Recommended controls: Undifferentiated human ES or EC cells stain weakly.



Immunoblotting:

Not tested



EIA:

Not tested



Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Research Sub Category
Neural Stem Cells

Biochem/physiol Actions

This monoclonal antibody recognizes a ganglioseries glycolipid, gangliosides GD3. Experimental evidence suggests that the expression of this antigen increases following human Embryonal Carcinoma and Embryonic Stem cell differentiation. It is thought that this antibody recognizes cells of the neuroectoderm lineage.

Physical form

Format: Purified
Purified immunoglobulin. Liquid in 0.05M Potassium Phosphate, 0.3M NaCl pH 8.0 with 0.05% Sodium Azide

Preparation Note

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 6 months after date of receipt.

Analysis Note

Control
Undifferentiated human embryonic stem or embryonic carcinoma cells stain weakly

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

分析证书(COA)

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Zhicheng Shao et al.
Nature communications, 7, 10869-10869 (2016-03-08)
It is well known that both recipient cells and donor nuclei demonstrate a mitotic advantage as observed in the traditional reprogramming with somatic cell nuclear transfer (SCNT). However, it is not known whether a specific mitotic factor plays a critical

相关内容

Human Stem Cell Systems

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

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