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Merck
CN

MAB4400

海肾萤光素酶抗体,克隆5B11.2

ascites fluid, clone 5B11.2, Chemicon®

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5B11.2, monoclonal
Application:
ELISA, Neutral, WB
Citations:
34
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biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

5B11.2, monoclonal

species reactivity (predicted by homology)

all

manufacturer/tradename

Chemicon®

technique(s)

ELISA: suitable, neutralization: suitable, western blot: suitable

isotype

IgG1

UniProt accession no.

shipped in

dry ice

target post-translational modification

unmodified

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General description

36 kDa
海肾萤光素酶是一种单体生物发光蛋白,来源于海肾(Renilla reniformis)。 在基因表达或相互作用的研究中,海肾荧光素酶常用作报告基因。36 kDa的酶催化底物腔肠素氧化为腔肠酰胺,从而产生可见光。海肾萤光素酶与萤火虫(Photalis pyralis)荧光素酶不同,识别的底物也不同,因此两种酶可以同时用于同一试验。

Immunogen

海肾萤光素酶GST融合蛋白

Application

可使用这种海肾萤光素酶抗体(克隆5B11.2)检测海肾萤光素酶,该抗体经验证可用于ELISA、FUNC、NEUT & WB。
研究子类别
表位标签
研究类别
表位标签 & 一般用途
蛋白质印迹:
先前批次的抗体已用于WB。

EIA:
先前批次的抗体已用于ELISA。

中和:
先前批次的抗体已用于NEAT。

最佳工作稀释度必须由最终用户确定。

Biochem/physiol Actions

海肾萤光素酶

Physical form

小鼠单克隆IgG1腹水。
未纯化

Preparation Note

自收到之日起,以未稀释的等分试样形式可于-20ºC条件下稳定保存1年。
处理建议:收到后,在取下瓶盖之前,将小瓶离心并轻轻混合溶液。分装到微量离心管中,并储存于-20°C。避免反复冻融循环,以免损坏IgG和影响产品性能。

Analysis Note

对照
海肾

Other Notes

浓度:请参考批次特异性浓缩物的检验报告。

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

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存储类别

11 - Combustible Solids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Maija Pollari et al.
PLoS pathogens, 16(10), e1008965-e1008965 (2020-10-09)
In this study, we demonstrate a novel pro-viral role for the Nicotiana benthamiana ARGONAUTE 1 (AGO1) in potyvirus infection. AGO1 strongly enhanced potato virus A (PVA) particle production and benefited the infection when supplied in excess. We subsequently identified the
Jaume Lillo et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Cannabinoids have been reported as orexigenic, i.e., as promoting food intake that, among others, is controlled by the so-called "hunger" hormone, ghrelin. The aim of this paper was to look for functional and/or molecular interactions between ghrelin GHSR1a and cannabinoid
Replication competent molecular clones of HIV-1 expressing Renilla luciferase facilitate the analysis of antibody inhibition in PBMC.
Edmonds, TG; Ding, H; Yuan, X; Wei, Q; Smith, KS; Conway, JA; Wieczorek, L; Brown et al.
Virology null
Yukichi Fujikawa et al.
The Plant journal : for cell and molecular biology, 52(1), 185-195 (2007-07-31)
We developed a split luciferase complementation assay to study protein-protein interactions in Arabidopsis protoplasts. In this assay, the N- and C-terminal fragments of Renilla reniforms luciferase are translationally fused to bait and prey proteins, respectively. When the proteins interact, split
Veronica Di Antonio et al.
Microorganisms, 9(5) (2021-05-01)
Human cytomegalovirus (HCMV) genome replication is a complex and still not completely understood process mediated by the highly coordinated interaction of host and viral products. Among the latter, six different proteins form the viral replication complex: a single-stranded DNA binding

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