Anti-Internexin α Antibody, CT, clone 2E3

The protein alpha-internexin is a ~66 kDa Class IV intermediate filament originally discovered as it copurifies with other neurofilament subunits {Pachter J et al J. Cell Biol 101:1316-22 (1985). It is related to but distinct from the better known neurofilament triplet proteins, NF-L, NF-M and NF-H, having similar protein sequence motifs and a similar intron organization. It is expressed in large amounts early in neuronal development, but is downregulated in many neurons as development procedes. Many classes of mature neurons contain alpha-internexin in addition to NF-L, NF-M anbd NF-H. In some mature neurons alpha-internexin is the only neurofilament subunit expressed. Antibodies to a-internexin are therefore unique probes to study and classify neuronal types and follow their processes in sections and in tissue culture. In addition the very early developmental expression of alpha-internexin means its presence is an early and convenient diagnostic feature of neuronal progenitors cells and other cell committed to the neuronal lineage. In addition recent studies show a marked up-regulation of a-internexin during neuronal regeneration {McGraw et al 2002}. The use of antibodies to this protein in the study of brain tumors has not been examined to date, but is likely to be of interest.

Epitope: C-terminus

Full length recombinant rat alpha internexin.

Research Category
Neuroscience

Research Sub Category
Neurofilament & Neuron Metabolism

Neuronal & Glial Markers

This Anti-Internexin Antibody, α, C-terminus, clone 2E3 is validated for use in ELISA, IP, WB, IH(P) for the detection of Internexin.

Western blot: antibody detects a protein band at 55-66kDa, as expected for alpha internexin {Evans, et al, 2001}.

Immunocytochemistry on PC12 and Ntera-2 cells.

Immunohistochemistry on formalin and methanol fixed tissues. Works on paraffin embedded tissue sections.

Immunoprecipitation

ELISA

Optimal working dilutions must be determined by the end user.

Alpha internexin. Localized to the C-terminal 164 amino acids of rat alpha internexin.

Mouse IgG in low serum (1%), tissue culture supernatant containing no preservative.

Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Replaces: 04-1032

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