conjugate
unconjugated
antibody form
purified antibody
clone
C14, monoclonal
purified by
using protein G
species reactivity
human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable, flow cytometry: suitable, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgGκ
UniProt accession no.
mode of action
| inhibits
target post-translational modification
unmodified
General description
54.60 kDa calculated.
GST-tagged full-length human recombinant Mucin-13.
Application
Inhibition Analysis: A representative lot inhibited pancreatic tumor growth in a pancreatic tumor xenograft mouse model (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
Western Blotting Analysis: A representative lot detected Mucin-13 in 293T MUC13 EGFP (transfected) versus 2093T EGFP (non-transfected) (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Mucin-13 in T84 and HPAFII membrane lysates (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
Immunocytochemistry Analysis: A represntative lot detected Mucin-13 in Capan-1, Panc-1, OMC3, SKOV-3, T84, SW480, HPAFII, and MiaPaca cells (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
Flow Cytometry Analysis: A represetative lot detected Mucin-13 in Capan-1, Panc-1, OMC3, SKOV-3, T84, SW480, HPAFII, and MiaPaca cells (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
ELISA Analysis: A representative lot detected Mucin-13 in Sandwich ELISA for the detection of MUC13 (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
Western Blotting Analysis: A representative lot detected Mucin-13 in 293T MUC13 EGFP (transfected) versus 2093T EGFP (non-transfected) (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
Immunoprecipitation Analysis: A representative lot immunoprecipitated Mucin-13 in T84 and HPAFII membrane lysates (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
Immunocytochemistry Analysis: A represntative lot detected Mucin-13 in Capan-1, Panc-1, OMC3, SKOV-3, T84, SW480, HPAFII, and MiaPaca cells (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
Flow Cytometry Analysis: A represetative lot detected Mucin-13 in Capan-1, Panc-1, OMC3, SKOV-3, T84, SW480, HPAFII, and MiaPaca cells (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
ELISA Analysis: A representative lot detected Mucin-13 in Sandwich ELISA for the detection of MUC13 (Courtesy of Subhash Chauhan, Ph.D., University of Tennessee Health Science Center, USA).
Biochem/physiol Actions
Clone C14 is a mouse monoclonal antibody that specifically detects human Mucin-13.
Physical form
Purified mouse monoclonal antibody IgG in PBS without azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Flow Cytometry in T84 cells.
Flow Cytometry Analysis: 1 µg of this antibody detected Mucin-13 in one million T84 colon adenocarcinoma cells.
Flow Cytometry Analysis: 1 µg of this antibody detected Mucin-13 in one million T84 colon adenocarcinoma cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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