Anti-FGFR4 Antibody, clone 3A11

Fibroblast growth factor receptor 4 (UniProt: P22455; also known as FGFR-4, CD334) is encoded by the FGFR4 (also known as JTK2, TKF) gene (Gene ID: 2264) in human. Fibroblast Growth Factor Receptor 4 (FGFR4) is a transmembrane tyrosine kinase receptor that plays a pivotal role in cellular homeostasis, development, and disease progression. FGFR4 consists of three extracellular immunoglobulin-like domains that mediate ligand binding, a transmembrane domain, and an intracellular domain with tyrosine kinase activity. FGFR4 is activated by fibroblast growth factors (FGFs), such as FGF19 (human homolog of FGF15), which initiates receptor dimerization and subsequent autophosphorylation of tyrosine residues, triggering downstream signaling cascades including MAPK, PI3K/AKT, and STAT pathways. FGFR4 is expressed in a tissue-specific manner, predominantly in the liver, pancreas, and adipose tissues, and is involved in bile acid metabolism, lipid regulation, and glucose homeostasis. It also has significant roles in muscle development and regeneration. Dysregulation of FGFR4, including overexpression or mutations, is associated with increased cell mobility, tumor progression, and poor prognosis in cancers such as breast, colon, and thyroid malignancies. In cancer, FGFR4 signaling contributes to tumorigenesis by promoting proliferation, survival, and angiogenesis. Mutations within the kinase domain of FGFR4 are linked to oncogenic activation, while truncated cytoplasmic isoforms exhibit transforming properties. FGFR4′s downstream signaling integrates with other pathways, such as WNT and TGF-β, adding complexity to its role in cancer biology. Its co-receptor, Klotho-beta (KLB), modulates FGFR4 activity, providing additional regulatory control over its signaling. (Ref.: Chen, X., et al., (2024), Eur J Med Chem. 263,115947)

Fc tagged recombinant protein fragment corresponding to the extracellular domain of human FGFR4.

Quality Control Testing

Evaluated by Immunocytochemistry in HepG2 cells.



Immunocytochemistry Analysis: A 1:250 dilution from a representative lot detected FGFR4 in HepG2 cells.

Immunotoxicity Assay: A representative lot detected FGFR4 in Immunotoxicity application (Patent US 2021/0324090 A1 2021); Tian, M., et al. (2023). Cell Rep Med. 4(10):101212; Tian, M., et al. (2024). Nat Commun. 15(1):6222; Xiao, W., et al. (2024). Cancer Gene Ther. 31(10):1571-1584).

Affinity Binding Assay: A representative lot detected FGFR4 in Affinity Binding Assay application (Tian, M., et al. (2023). Cell Rep Med. 4(10):101212).

Flow Cytometry: A representative lot detected FGFR4 in Flow Cytometry application (Patent US 2021/0324090 A1 2021) ; Tian, M., et al. (2023). Cell Rep Med. 4(10):101212; Tian, M., et al. (2024). Nat Commun. 15(1):6222; Xiao, W., et al. (2024). Cancer Gene Ther. 31(10):1571-1584).

Immunohistochemistry: A representative lot detected FGFR4 in Immunohistochemistry application (Patent US 2021/0324090 A1 2021) ; Tian, M., et al. (2023). Cell Rep Med. 4(10):101212; Tian, M., et al. (2024). Nat Commun. 15(1):6222.; Xiao, W., et al. (2024). Cancer Gene Ther. 31(10):1571-1584).

ELISA: A representative lot detected FGFR4 in ELISA application (Patent US 2021/0324090 A1 2021); Tian, M., et al. (2023). Cell Rep Med. 4(10):101212).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 3A11 is a mouse monoclonal antibody that detects FGFR4. It targets an epitope from the extracellular domain.

Purified mouse monoclonal antibody IgG2b in PBS without preservatives.

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.