Anti-Rheb, clone 3H6

Anti-Rheb, clone 3H6, Cat. No. MABC1605, is a mouse monoclonal antiboody that detects GTP-binding protein Rheb and has been tested for use in Western Blotting.

Western Blotting

Western Blotting Analysis: A representative lot detected Rheb in Western Blotting applications (Findlay, G.M., et. al. (2007). Biochem J. 403(1):13-20; Groenewoud, M.J., et. al. (2013). PLoS One. 8(11):e81649.

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected Rheb in MCF-7, JEG-3, and HT-29 cell lysates.

Clone 3H6 is a mouse monoclonal antibody that specifically detects human GTP-binding protein Rheb.

GST-tagged recombinant fragment corresponding to the first 167 amino acids of human GTP-binding protein Rheb.

Stable for 1 year at 2-8°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Purified mouse monoclonal antibodyIgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Western Blotting in lysate from DLD-1 cells.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Rheb in lysate from DLD-1 cells.

GTP-binding protein Rheb (UniProt: Q15382; also known as Ras homolog enriched in brain) is encoded by the RHEB (also known as RHEB2) gene (Gene ID: 6009) in human. Rheb is a highly conserved small GTPase with low intrinsic GTPase activity. It is ubiquitous in its distribution, but higher levels of Rheb are reported in the brain, and in skeletal and cardiac muscles. It is synthesized with a short propeptide (aa 182-184), which is cleaved off to generate the active form. It is considered as a critical regulator of mTORC1 activity and is shown to bind to mTORC1 in a guanyl nucleotide-independent manner. It stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Rheb is regulated in a negative manner by the GTPase-activating protein activity of a complex consisting of tuberous sclerosis complex 1 (TSC1) and TSC2. Akt or ERK1/2 are reported to phosphorylate and inactivate TSC that leads to the activation of Rheb and mTORC1. (Ref.: Findlay, GM et al. (2007) Biochem. J. 403(1); 13-20).

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.