Anti-csBAP31, clone 144-A8

Anti-csBAP31, clone 144-A8, Cat. No. MABC1710, is a mouse monoclonal antibody that detects B-cell receptor-associated protein 31 and has been tested for use in ELISA, Flow Cytometry, Immunoprecipitation, and Western Blotting.

ELISA Flow Cytometry Immunoprecipitation Western Blotting

Immunoprecipitation Analysis: A representative lot immunoprecipitated csBAP31 in Immunoprecipitation applications (Kim, WT., et. al. (2017). PLoS One. 12(1):e0170145).

Western Blotting Analysis: A representative lot detected csBAP31 in Western Blotting applications (Kim, WT., et. al. (2016). PLoS One. 11(12):e0167527; Kim, WT., et. al. (2017). PLoS One. 12(1):e0170145).

ELISA Analysis: A representative lot detected csBAP31 in ELISA applications (Kim, WT., et. al. (2016). PLoS One. 11(12):e0167527).

Flow Cytometry Analysis: A representative lot detected csBAP31 in Flow Cytometry applications (Kim, WT., et. al. (2016). PLoS One. 11(12):e0167527; Kim, WT., et. al. (2017). PLoS One. 12(1):e0170145).

Clone 144-A8 is a mouse monoclonal antibody that detects human Cell surface B-cell receptor-associated protein 31 (csBAP31). It targets an epitope within 10 amino acids from the C-terminal region.

H9 human embryonic stem cells pretreated with retinoic acid.

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

B-cell receptor-associated protein 31 (UniProt: P51572; also known as BCR-associated protein 31, BAP31, 6C6-AG tumor-associated antigen, Protein CDM, p28) is encoded by the BCAP31 (also known as BAP31, DXS1357E) gene (Gene ID: 10134) in human. BAP31 is an endoplasmic reticulum (ER) membrane protein that is also expressed on surface (csBAP31) of embryonic stem cells. ER associated BAP1 palsy a significant role as a molecular chaperone for the newly synthesized transmembrane proteins and also acts as an important regulator of apoptosis. It interacts with pro-caspase-8L and Bcl-2/Bcl-xL on the ER membrane and is cleaved by caspase-8. The resulting cleaved product, p20, is an important inducer of apoptosis. The cell surface expressed BAP31 is shown to promote cell survival through the regulation of cell adhesion to the extracellular matrix in human embryonic stem cells. csBAP31 is also suggested to be a putative pro-apoptotic flag on cancer cells. Its expression is shown to be enhanced in caspase3/7 high A549 cells and csBAP31-positive cells display diminished survival capacity. Higher levels of csBAP31 are reported to contribute to poor survival cancer cells. Clone SP1-B7 specifically recognizes the cell surface-expressed BAP31. (Ref.: Seo, SR et al. (2017). PLoS One 12(11): e0188075).

Evaluated by Flow Cytometry in Ramos cells.

Flow Cytometry Analysis: 1 µg of this antibody detected csBAP31 in one million Ramos cells.

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