conjugate
unconjugated
antibody form
purified antibody
clone
297-D4, monoclonal
purified by
using protein G
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable, flow cytometry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
species reactivity
mouse
General description
27.99 kDa calculated.
H9 human embryonic stem cells.
Application
ELISA Analysis: A representative lot detected csBAP31 in ELISA applications (Kim, WT., et. al. (2016). PLoS One. 11(12):e0167527).
Flow Cytometry Analysis: A representative lot detected csBAP31 in Flow Cytometry applications (Kim, WT., et. al. (2016). PLoS One. 11(12):e0167527; Kim, WT., et. al. (2017). PLoS One. 12(1):e0170145).
Immunoprecipitation Analysis: A representative lot immunoprecipitated csBAP31 in Immunoprecipitation applications (Kim, WT., et. al. (2017). PLoS One. 12(1):e0170145).
Western Blotting Analysis: A representative lot detected csBAP31 in Western Blotting applications (Kim, WT., et. al. (2016). PLoS One. 11(12):e0167527; Kim, WT., et. al. (2017). PLoS One. 12(1):e0170145).
Flow Cytometry Analysis: A representative lot detected csBAP31 in Flow Cytometry applications (Kim, WT., et. al. (2016). PLoS One. 11(12):e0167527; Kim, WT., et. al. (2017). PLoS One. 12(1):e0170145).
Immunoprecipitation Analysis: A representative lot immunoprecipitated csBAP31 in Immunoprecipitation applications (Kim, WT., et. al. (2017). PLoS One. 12(1):e0170145).
Western Blotting Analysis: A representative lot detected csBAP31 in Western Blotting applications (Kim, WT., et. al. (2016). PLoS One. 11(12):e0167527; Kim, WT., et. al. (2017). PLoS One. 12(1):e0170145).
Biochem/physiol Actions
Clone 297-D4 is a mouse monoclonal antibody that detects human Cell surface B-cell receptor-associated protein 31 (csBAP31). It targets an epitope within 10 amino acids from the C-terminal region.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Flow Cytometry in Ramos cells.
Flow Cytometry Analysis: 1 µg of this antibody detected csBAP31 in one million Ramos cells.
Flow Cytometry Analysis: 1 µg of this antibody detected csBAP31 in one million Ramos cells.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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