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Merck
CN

MABC1751

Anti-KISS1 Antibody, clone 6A4.27

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UNSPSC Code:
12352203
NACRES:
NA.43
eCl@ss:
32160702
Species reactivity:
human, mouse
Conjugate:
unconjugated
Application:
IHC, IP, WB
Citations:
-
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conjugate

unconjugated

antibody form

purified antibody

clone

6A4.27, monoclonal

purified by

using protein G

species reactivity

human, mouse

concentration

(Please refer to lot specific datasheet.)

technique(s)

immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable

isotype

IgG1κ

UniProt accession no.

target post-translational modification

unmodified

General description

14.70 kDa calculated.
A linear peptide corresponding to 54 amino acids from the C-terminal half of human Kisspeptin-1 (KISS1).

Application

Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected KISS1 in human pancreatic cancer tissue sections.

Immunohistochemistry (Paraffin) Analysis: A representative lot detected KISS1 in Immunohistochemistry applications (McNally, L.R., et. al. (2010). Clin Exp Metastasis. 27(8):591-600; Ulasov, I.V., et. al. (2012). Cancer. 118(8):2096-105).

Western Blotting Analysis: A representative lot detected KISS1 in Western Blotting applications (Harihar, S., et. al. (2014). PLoS One. 9(1):e84958).

Immunoprecipitation Analysis: A representative immunoprecipitated KISS1 in Immunoprecipitation applications (Harihar, S., et. al. (2014). PLoS One. 9(1):e84958).

Biochem/physiol Actions

Clone 6A4.27 is a mouse monoclonal antibody that detects Kisspeptin-1. It targets an epitope with in the KP54 (G68-F121) region.

Physical form

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Evaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected KISS1 in human placenta tissue sections.

Other Notes

Human, Mouse.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


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