conjugate
unconjugated
antibody form
purified antibody
clone
6A4.27, monoclonal
purified by
using protein G
species reactivity
human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
immunohistochemistry: suitable, immunoprecipitation (IP): suitable, western blot: suitable
isotype
IgG1κ
UniProt accession no.
target post-translational modification
unmodified
General description
14.70 kDa calculated.
A linear peptide corresponding to 54 amino acids from the C-terminal half of human Kisspeptin-1 (KISS1).
Application
Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution from a representative lot detected KISS1 in human pancreatic cancer tissue sections.
Immunohistochemistry (Paraffin) Analysis: A representative lot detected KISS1 in Immunohistochemistry applications (McNally, L.R., et. al. (2010). Clin Exp Metastasis. 27(8):591-600; Ulasov, I.V., et. al. (2012). Cancer. 118(8):2096-105).
Western Blotting Analysis: A representative lot detected KISS1 in Western Blotting applications (Harihar, S., et. al. (2014). PLoS One. 9(1):e84958).
Immunoprecipitation Analysis: A representative immunoprecipitated KISS1 in Immunoprecipitation applications (Harihar, S., et. al. (2014). PLoS One. 9(1):e84958).
Immunohistochemistry (Paraffin) Analysis: A representative lot detected KISS1 in Immunohistochemistry applications (McNally, L.R., et. al. (2010). Clin Exp Metastasis. 27(8):591-600; Ulasov, I.V., et. al. (2012). Cancer. 118(8):2096-105).
Western Blotting Analysis: A representative lot detected KISS1 in Western Blotting applications (Harihar, S., et. al. (2014). PLoS One. 9(1):e84958).
Immunoprecipitation Analysis: A representative immunoprecipitated KISS1 in Immunoprecipitation applications (Harihar, S., et. al. (2014). PLoS One. 9(1):e84958).
Biochem/physiol Actions
Clone 6A4.27 is a mouse monoclonal antibody that detects Kisspeptin-1. It targets an epitope with in the KP54 (G68-F121) region.
Physical form
Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Preparation Note
Stable for 1 year at 2-8°C from date of receipt.
Analysis Note
Evaluated by Immunohistochemistry (Paraffin) in human placenta tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected KISS1 in human placenta tissue sections.
Immunohistochemistry (Paraffin) Analysis: A 1:250 dilution of this antibody detected KISS1 in human placenta tissue sections.
Other Notes
Human, Mouse.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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