Anti-Cullin-4B/CUL4B Antibody, clone 9B11.1

The protein CUL4B acts as the scaffold protein within the multi-protein RING E3 ubiquitin protein ligase complexes needed for ubiquitination and subsequent proteasomal degradation of target proteins. CUL4B appears to contribute to the catalysis of ubiquitination by positioning the substrate target protein and the E3 ubiquitin ligase. Among the targets CUL4B is associated with include histone H2A, H3, p53, cyclin E and H4. The cell signaling mTORC1 pathway is also regulated via CUL4b interaction with MLST8 a key player in the mTORC1 pathway. Thus CUL4B is important is proper cell division, and cell cycle function, and is also associated with tumorigenesis in cancer by coordinating with various methylases in promoting specific DNA methylation-based epigenetic silencing in certain cancers. CUL4B is localized in the nucleus as well as the cytoplasm depending upon the context and is widely expressed.

~104 kDa observed

GST-tagged recombinant protein corresponding to human Cullin-4B/CUL4B.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected Cullin-4B/CUL4B in human kidney tissue.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

This Anti-Cullin-4B/CUL4B Antibody, clone 9B11.1 is validated for use in Western Blotting and Immunohistochemistry for the detection of Cullin-4B/CUL4B.

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in A549 cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Cullin-4B/CUL4B in 10 µg of A549 cell lysate.

Concentration: Please refer to lot specific datasheet.

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