Anti-HsSAS-6 Antibody, clone 91.390.21

Spindle assembly abnormal protein 6 homolog (UniProt: Q6UVJ0; also known as HsSAS-6) is encoded by the SASS6 (also known as SAS6) gene (Gene ID: 163786) in human. HsSAS-6 is a central scaffolding component of the centrioles that ensure their 9-fold symmetry. It functions to ensure that each centriole seeds the formation of a single procentriole per cell cycle. It is required for centrosome biogenesis and duplication and is essential for both for mother-centriole-dependent centriole duplication and deuterosome-dependent centriole amplification in multi-ciliated cells. It contains a 35 nM long coiled-coil domain that mediates homodimerization while the globular N-terminus links the dimers at an angle of 40 degrees to form the inner ring. Over-expression of SASS6 gene results in excess foci-bearing centriolar markers. Mutations in SASS6 are known to cause microcephaly 14 that is characterized by small head circumference, markedly reduced brain weight and disproportionately small cerebral cortex.

~74 kDa observed; 74.40 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Epitope: C-terminus

His-tagged recombinant fragment corresponding to 254 amino acids from the C-terminal half of human Spindle assembly abnormal protein 6 homolog (HsSAS-6).

Anti-HsSAS-6, clone 91.390.21, Cat. No. MABE1064, is a highly specific mouse monoclonal antibody that targets SASS6 and has been tested in Immunofluorescence, Immunoprecipitation, and Western Blotting.

Research Category
Epigenetics & Nuclear Function

Western Blotting Analysis: 1 µg/mL from a representative lot detected HsSAS-6 in 10 µg of HeLa cell lysate.

Western Blotting Analysis: A representative lot detected HsSAS-6 in RNAi in U2OS cell lysates (Courtesy of Professor Dr. Erich Nigg, Ph.D; Biozentrum, University of Basel, Switzerland).

Immunofluorescence Analysis: A representative lot detected HsSAS-6 in RNAi followed by immunofluorescence (Courtesy of Professor Dr. Erich Nigg, PhD; Biozentrum, University of Basel, Switzerland).

Immunofluorescence Analysis: A representative lot detected HsSAS-6 in Immunofluorescence applications (Kleylein-Sohn, J. et. al. (2007). Dev Cell. 13(2):190-202; Sonnen, K..,et. al. (2012). Biol Open 1(10):965-76; Arquint, C., et al. (2012). J Cell Sci. 125(Pt 5):1342-52).

Immunoprecipitation Analysis: A representative lot detected HsSAS-6 in RNAi followed by immunoprecipitation (Courtesy of Professor Dr. Erich Nigg, PhD; Biozentrum, University of Basel, Switzerland).

Clone 91.390.21 detects HsSAS-6 protein in human cells. It targets an epitope with in 254 amino acids in the C-terminal half.

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in U20S cell lysate.

Western Blotting Analysis: 1 µg/mL of this antibody detected HsSAS-6 in 10 µg of U2OS cell lysate.

Concentration: Please refer to lot specific datasheet.

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