生物来源
mouse
质量水平
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
1H6, monoclonal
种属反应性(根据同源性预测)
all
技术
ELISA: suitable
flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
同位素/亚型
IgG2bκ
运输
wet ice
靶向翻译后修饰
unmodified
一般描述
免疫原
应用
免疫细胞化学分析:一个代表性批次在中期HeLa和鼠胚胎干细胞的致密染色体上检测到离散的DNA G-四链体(G4)聚焦点(Henderson,A.,et al.(2014).Nucleic Acids Res. 42(2):860-869)。
免疫细胞化学分析:在DNase处理低聚甲醛固定和Triton X-100透性化HeLa细胞后,一个代表性批次检测到核DNA G-四链体(G4)免疫反应性的大幅度降低(Henderson,A.,et al.(2014).Nucleic Acids Res. 42(2):860-869)。
免疫细胞化学分析:一个代表性批次在缺乏G4解旋解旋酶FANCJ的鸡DT40细胞中检测到G-四链体(G4)稳定剂端粒抑素/TMS处理诱导的核DNA G4免疫反应性,而在表达人FANCJ的DT40细胞中未观察到药物增强作用(Henderson,A.,et al.(2014).Nucleic Acids Res. 42(2):860-869)。
免疫细胞化学分析:在DNA G4稳定剂端粒抑素/TMS处理后,一个代表性批次检测到U20S细胞中核DNA G-四链体(G4)聚焦点呈时间依赖性增加(Henderson,A.,et al.(2014).Nucleic Acids Res. 42(2):860-869)。
ELISA分析:通过竞争性ELISA测定代表性批次的目标选择性。克隆1H6结合四分子和单分子(分子内)DNA G-四链体(G4)结构(Henderson,A.,et al.(2014).Nucleic Acids Res. 42(2):860-869)。
流式细胞术分析:在用DNA G4稳定剂TMPyP4(目录号613560)处理后,一个代表性批次在HeLa细胞中检测到增强的DNA G-四链体(G4)免疫反应性(Henderson,A.,et al.(2014).Nucleic Acids Res. 42(2):860-869)。
免疫组织化学分析:一个代表性批次在各种石蜡包埋的人体组织切片中检测到核DNA G-四链体(G4)免疫反应性,包括皮肤、胰腺、睾丸、胎盘、脑、阑尾、结肠和肾脏(Henderson,A.,et al.(2014).Nucleic Acids Res. 42(2):860-869)。
核受体
表观遗传学&核功能
生化/生理作用
外形
制备说明
分析说明
免疫细胞化学分析:2.0 µg/mL该抗体可在HeLa细胞中检测到DNA G-四链体(G4)。
其他说明
免责声明
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储存分类代码
12 - Non Combustible Liquids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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