Anti-RING2/RNF2 Antibody, Clone 3-3

GST-tagged full length murine RING2 protein.

~42 kDa observed; 37.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Immunoprecipitation Analysis: A representative lot immunoprecipitated RING2/RNF2 in Immunoprecipitation applications (Yakushiji-Kaminatsui, N., et. al. (2016). Development. 143(2):276-85; Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6; Endoh, M., et. al. (2017). Elife. 6. pii: e27970).

Immunocytochemistry Analysis: A representative lot detected RING2/RNF2 in Immunocytochemistry applications (Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6).

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected RING2/RNF2 in Chromatin Immunoprecipitation applications (Farcas, A.M., et. al. (2012). Elife. 1:e00205; Endoh, M., et. al. (2017). Elife. 6. pii: e27970).

Western Blotting Analysis: A representative lot detected RING2/RNF2 in Western Blotting applications (Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6).

Clone 3-3 is a mouse monoclonal antibody that specifically detects E3 ubiquitin-protein ligase RING2 (RING1B).

Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in U20S cell lysates.

Western Blotting Analysis: 1 µg/mL of this antibody detected RING2/RNF2 in U20S cell lysate.

Human, Monkey, Mouse.

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