偶联物
unconjugated
抗体形式
purified antibody
克隆
3-3
monoclonal
纯化方式
using protein G
种属反应性
mouse, human, human, monkey, mouse, monkey, mouse
浓度
(Please refer to lot specific datasheet.)
技术
ChIP: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型
IgG2bκ
UniProt登记号
靶向翻译后修饰
unmodified
一般描述
GST-tagged full length murine RING2 protein.
~42 kDa observed; 37.62 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
应用
Immunoprecipitation Analysis: A representative lot immunoprecipitated RING2/RNF2 in Immunoprecipitation applications (Yakushiji-Kaminatsui, N., et. al. (2016). Development. 143(2):276-85; Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6; Endoh, M., et. al. (2017). Elife. 6. pii: e27970).
Immunocytochemistry Analysis: A representative lot detected RING2/RNF2 in Immunocytochemistry applications (Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6).
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected RING2/RNF2 in Chromatin Immunoprecipitation applications (Farcas, A.M., et. al. (2012). Elife. 1:e00205; Endoh, M., et. al. (2017). Elife. 6. pii: e27970).
Western Blotting Analysis: A representative lot detected RING2/RNF2 in Western Blotting applications (Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6).
Immunocytochemistry Analysis: A representative lot detected RING2/RNF2 in Immunocytochemistry applications (Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6).
Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected RING2/RNF2 in Chromatin Immunoprecipitation applications (Farcas, A.M., et. al. (2012). Elife. 1:e00205; Endoh, M., et. al. (2017). Elife. 6. pii: e27970).
Western Blotting Analysis: A representative lot detected RING2/RNF2 in Western Blotting applications (Atsuta, T., et. al. (2001). Hybridoma. 20(1):43-6).
生化/生理作用
Clone 3-3 is a mouse monoclonal antibody that specifically detects E3 ubiquitin-protein ligase RING2 (RING1B).
外形
Purified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
制备说明
Stable for 1 year at 2-8°C from date of receipt.
分析说明
Evaluated by Western Blotting in U20S cell lysates.
Western Blotting Analysis: 1 µg/mL of this antibody detected RING2/RNF2 in U20S cell lysate.
Western Blotting Analysis: 1 µg/mL of this antibody detected RING2/RNF2 in U20S cell lysate.
其他说明
Human, Monkey, Mouse.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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