Anti-ERR alpha, clone 2ERR10

Anti-ERR alpha, clone 2ERR10, Cat. No. MABE1891, is a mouse monoclonal antibody that detects Steroid hormone receptor ERR alpha and has been tested for use in Chromatin Immunoprecipitation, ELISA, Immunocytochemistry, Immunoprecipitation, and Western Blotting.

Chromatin Immunoprecipitation (ChIP) ELISA Immunocytochemistry Immunoprecipitation Western Blotting

Immunocytochemistry Analysis: A 1:20 dilution from a representative lot detected ERR alpha in MCF-7 cells.

Immunoprecipitation Analysis: A representative lot immunoprecipitated ERR alpha in Immunoprecipitation applications (Esch, A.M., et. al. (2012). Protein Expr. Purif. 84(1):47-58).

Immunocytochemistry Analysis: A representative lot detected ERR alpha in Immunocytochemistry applications (Esch, A.M., et. al. (2012). Protein Expr. Purif. 84(1):47-58).

Western Blotting Analysis: A representative lot detected ERR alpha in Western Blotting applications (Esch, A.M., et. al. (2012). Protein Expr. Purif. 84(1):47-58).

Chromatin Immunoprecipitation Analysis (ChIP): A representative lot detected ERR alpha in Chromatin Immunoprecipitation applications (Esch, A.M., et. al. (2012). Protein Expr. Purrif. 84(1):47-58).

Enzyme Immunoassay Analysis: A representative lot detected ERR alpha in ELISA applications (Esch, A.M., et. al. (2012). Protein Expr. Purif. 84(1):47-58).

Clone 2ERR10 is a mouse monoclonal antibody that detects human Steroid hormone receptor ERR alpha (ERR1).

His-tagged full length human recombinant Steroid hormone receptor ERR alpha (ERR1).

Stable for 1 year at 2-8°C from date of receipt.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Evaluated by Western Blotting in lysate from HEK293T cells transfected with ERR alpha.

Western Blotting Analysis: 1 µg/mL of this antibody detected ERR alpha in lysate from HEK293T transfected with ERR alpha.

Steroid hormone receptor ERR1 (UniProt: P11474; also known as Estrogen receptor-like 1, Estrogen-related receptor alpha, ERR-alpha, Nuclear receptor subfamily 3 group B member 1) is encoded by the ESRRA (also known as ERR1, ESRL1, NR3B1) gene (Gene ID: 2101) in human. ERR1 is a orphan nuclear receptor with no known natural ligands. Its transcriptional activities are thought to be modulated by post-translational modifications and interactions with cofactors. It is localized predominantly in cell nuclei but can co-localize to the cytoplasm in presence of MAPK15. It is most highly expressed in skeletal muscle, kidney, heart, brain, and intestine. ERR1 contains four major canonical nuclear receptor domains. The N-terminal A/B domain (aa 1-78) is involved in ligand-independent functions of nuclear receptors. The C region (aa 79-144) contains the DNA-binding domain (DBD)and adjacent to the DBD is the D domain (aa 145-198), which is also known as the hinge region. The C-terminal E/F domain (aa 199-423) typically contains a ligand-binding region for most nuclear receptors. ERR1 contains two NR C-type zinc finger domains (aa 79-99 and 115-134). Its activity is induced by PGC1 alpha in several specific cell types. ERR1 regulates genes involved in mitochondrial biogenesis, gluconeogenesis, oxidative phosphorylation, and fatty acid metabolism, and brown adipose tissue thermogenesis. Phosphorylation of ERR1 at serine 19 is shown to enhance its sumolyation on lysine 14, which can increase repression of its transcriptional activity. Over-expression of ERR1 has been linked to breast, colon, and ovarian cancers. (Ref.: Esch, AM., et al. (2012). Protein Expression and Purification 84(1); 47-58).

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