Anti-DDX3 Antibody, clone AO196

ATP-dependent RNA helicase DDX3X (UniProt: O00571; also known as EC:3.6.4.13, CAP-Rf, DEAD box protein 3 X-chromosomal, DEAD box X isoform, DBX, Helicase-like protein 2, HLP2) is encoded by the DDX3X (also known as DBX, DDX3) gene (Gene ID: 1654) in human. DDX3 is a ubiquitous cellular protein that is widely expressed in mammalian cells and is distributed throughout the cytoplasm and can also be found in the nucleus. It shuttles between the nucleus and the cytosol and is exported from the nucleus partly through the XPO1/CRM1 system and partly through NXF1/TAP. It serves as a multifunctional ATP-dependent RNA helicase and also displays ATPase activity that can be stimulated by various ribo-and deoxynucleic acids. It is also involved in many cellular processes, which do not necessarily require its ATPase/helicase catalytic activities. It is also involved in positive regulation of CDKN1A/p21 transcription in an SP1-dependent manner, hence can inhibit cell growth. This function of DDX3 requires its ATPase, but not the helicase activity. Independently of its ATPase activity, it can also promote the assembly of functional 80S ribosomes and disassembles from ribosomes prior to the translation elongation process. Although it is not involved in the general process of translation but is shown to promote efficient translation of selected complex mRNAs, containing highly structured 5′-untranslated regions (UTR). This function or DDX3 is dependent on its helicase activity. DDX3 is also involved in innate immunity, acting as a viral RNA sensor. It binds viral RNAs and promotes the production of interferon a and b . The cellular DDX3 is shown to be essential for hepatitis C virus (HCV) and HIV-1 replication. However, it inhibits hepatitis B virus transcription by its incorporation into nucleocapsids. During HCV infection, HCV core protein is reported to inhibit the interaction between MAVS and DDX3 and thus impairs MAVS-dependent INF- b induction. DDX3 can undergo phosphorylation by TBK1 and this phosphorylation is required for the synergistic induction of IFN-b mediated by TBK1 and DDX3. (Ref.: Geissler, R., et al. (2012). Nucl. Acid. Res. 40(11); 4998-5011; Angus, AGN., et al. (2010). J. Gen. Virol. 91; 122-132; Soulat, D., et al. (2008). EMBO J. 27(15); 2135-2146).

GST-tagged recombinant fragment corresponding to the first 142 amino acids from the N-terminal region of human ATP-dependent RNA helicase DDX3X (DDX3).

Quality Control Testing

Evaluated by Western Blotting in MCF-7 cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected DDX3 in MCF-7 cell lysate.

Tested Applications

Western Blotting Analysis: A 1:500 dilution from a representative lot detected DDX3 in MDA-MB-231 cell lysate.

Immunofluorescence Analysis: A representative lot detected DDX3 in Immunofluorescence applications (Heerma van Voss, M.R., et al. (2018). Oncogene. 37(1):63-74).

Immunoprecipitation Analysis: A representative lot immunoprecipitated DDX3 in Immunoprecipitation applications (Angus, A.G., et al. (2010). J Gen Virol. 91(Pt 1):122-32).

Immunocytochemistry Analysis: A representative lot detected DDX3 in Immunocytochemistry applications (Angus, A.G., et al. (2010). J Gen Virol. 91(Pt 1):122-32).

Western Blotting Analysis: A representative lot detected DDX3 in Western Blotting applications (Angus, A.G., et al. (2010). J Gen Virol. 91(Pt 1):122-32; Heerma van Voss, M.R., et al. (2018). Oncogene. 37(1):63-74).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Anti-DDX3, clone AO196, Cat. No. MABE1921, is a mouse monoclonal antibody that detects ATP-dependent RNA helicase DDX3 and is tested for use in Immunocytochemistry, Immunofluorescence, Immunoprecipitation, and Western Blotting.

Clone AO196 is a mouse monoclonal antibody that detects ATP-dependent RNA helicase DDX3. It targets an epitope within the N-terminal region.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Recommend storage at +2°C to +8°C. For long term storage antibodies can be kept at -20°C. Avoid repeated freeze-thaws.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.