Anti-SLUG/SNAI2 Antibody, clone 62.1E6

Zinc finger protein SNAI2 (UniProt: Q91051; also known as Protein snail homolog 2) is encoded by the Slug gene (Gene ID: 432368) in chicken. All members of the snail gene family are zinc-finger transcription factors expressed early in embryonic development and are involved in the formation of tissues such as mesoderm and presumptive neural crest. SLUG is a transcriptional repressor that modulates both activator-dependent and basal transcription. In humans, SLUG (UniProt: O43623; also known as Neural crest transcription factor Slug) is encoded by the SNAI2 (also known as SLUG, SLUGH) gene (Gene ID: 6591). It is expressed in most adult human tissues, but is not detected in peripheral blood leukocyte. It is observed in discrete foci in interphase nuclei and these nuclear foci do not overlap with the nucleoli, the SP100 and the HP1 heterochromatin or the coiled body, suggesting its association with active transcription or active splicing regions. It is also shown to repress BRCA2 expression by binding to its E2-box-containing silencer and recruiting CTBP1 and HDAC1 in breast cells. SLUG is shown to be involved in the generation and migration of neural crest cells. It also plays a role in mediating RAF1-induced transcriptional repression of the TJ protein, occludin (OCLN) and subsequent oncogenic transformation of epithelial cells. Mutation in SNAI2 gene can cause Waardenburg syndrome 2D that is characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. This 62.1E6 mouse monoclonal antibody has been validated in Immunohistochemistry, Immunofluorescence, Chromatin Immunoprecipitation. (Ref.:Perez-Alcala, S., et al. (2004). Development. 131(18):4455-65; Jhingory, S., et al. (2010). Dev Biol. 344(2):896-910; Martinez-Morales, P.L., et al. (2011). J Cell Biol. 194(3):489-503; Endo, Y., et al. (2012). Dev Cell. 23(2):305-16; Shoshkes-Carmel, M., et al. (2015). PLoS One. 10(8):e0136408).

His-tagged, full Length, recombinant chicken SLUG/SNAI2.

Quality Control Testing

Evaluated by Western Blotting with recombinant human SLUG/SNAI2.

Western Blotting Analysis: A 1:500 dilution from a representative lot detected His-tagged, full length, recombinant human SLUG/SNAI2.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Slug/SNAI2 in Immunofluorescence application (Shoshkes-Carmel, M., et al. (2015). PLoS One. 10(8):e0136408).

Chromatin Immunoprecipitation (ChIP) Analysis: A representative lot detected Slug/SNAI2 in Chromatin Immunoprecipitation application (Jhingory, S., et al. (2010). Dev Biol. 344(2):896-910).

Immunohistochemistry Applications: A representative lot detected SLUG/SNAI2 in Immunohistochemistry applications (Perez-Alcala, S., et al. (2004). Development. 131(18):4455-65; Jhingory, S., et al. (2010). Dev Biol. 344(2):896-910; Martinez-Morales, P.L., et al. (2011). J Cell Biol. 194(3):489-503; Endo, Y., et al. (2012). Dev Cell. 23(2):305-16; Shoshkes-Carmel, M., et al. (2015). PLoS One. 10(8):e0136408; Li, J., et al. (2019). Sci Rep. 9(1):11191).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Clone 62.1E6 is a mouse monoclonal antibody that specifically detects SLUG/SNAI2.

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Recommended storage: +2°C to +8°C.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.